Fernandez-Novo Aitor, Santos-Lopez Sergio, Barrajon-Masa Clara, Mozas Patricia, de Mercado Eduardo, Caceres Elisa, Garrafa Aizic, Gonzalez-Martin Juan Vicente, Perez-Villalobos Natividad, Oliet Agustin, Astiz Susana, Perez-Garnelo Sonia Salome
Department of Veterinary Medicine, School of Biomedical and Health Sciences, Universidad Europea de Madrid, C/Tajo s/n, Villaviciosa de Odón, 28670 Madrid, Spain.
Animal Production Department, Veterinary Faculty, Complutense University of Madrid, Puerta de Hierro Avenue s/n, 28040 Madrid, Spain.
Biology (Basel). 2021 Jul 7;10(7):630. doi: 10.3390/biology10070630.
Seminal parameters can be evaluated in situ, or samples can be delivered to a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0-2 h, 4-6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed, BIOXcell or INRA96) on semen quality. Acrosome integrity, sperm viability and morphology, CASA-total and progressive motility, pH, and colony-forming units were assessed. Semen quality was preserved for up to 4-6 h post-ejaculation, except for INRA96 at 5 °C. Regardless of extender or temperature, motility decreased from 4 to 6 h up to 24 h, with the best values obtained with BIOXcell at 5 °C. pH differed from 4 to 6 h up to 24 h, acidifying when stored at room temperature. Microbiological load was stable over time with AndroMed and BIOXcell, and increased at room temperature with INRA96. Our results suggest that AndroMed and BIOXcell can preserve semen quality for up to 6 h, either at 5 °C or room temperature, while INRA96 only at room temperature. These results help to fix adequate protocols for short-term storage and shipment of bovine semen collected under field conditions.
精液参数可在现场进行评估，也可将样本送到诊断中心。在评估前储存条件如何影响射精样本尚不清楚。我们评估了25头在野外通过电刺激采精的商用公牛的精液，评估了射精后至评估的时间（0 - 2小时、4 - 6小时和24小时）、保存温度（5°C与室温）以及稀释液（AndroMed、BIOXcell或INRA96）对精液质量的影响。评估了顶体完整性、精子活力和形态、计算机辅助精子分析（CASA）的总活力和渐进性活力、pH值以及菌落形成单位。射精后精液质量可保存长达4 - 6小时，但5°C下的INRA96稀释液除外。无论稀释液或温度如何，活力在4至6小时到24小时之间下降，5°C下的BIOXcell稀释液获得的活力值最佳。pH值在4至6小时到24小时之间有所不同，在室温下储存时会酸化。使用AndroMed和BIOXcell时微生物负荷随时间稳定，而在室温下使用INRA96时微生物负荷会增加。我们的结果表明，AndroMed和BIOXcell在5°C或室温下均可将精液质量保存长达6小时，而INRA96仅在室温下可行。这些结果有助于确定在野外条件下采集的牛精液短期储存和运输的适当方案。
