Siepenkoetter Till, Salaj-Kosla Urszula, Xiao Xinxin, Conghaile Peter Ó, Pita Marcos, Ludwig Roland, Magner Edmond
Department of Chemical Sciences, Bernal Institute, University of Limerick, Limerick, Ireland.
School of Chemistry, Ryan Institute, National University of Ireland, Galway, Ireland.
Chempluschem. 2017 Apr;82(4):553-560. doi: 10.1002/cplu.201600455. Epub 2016 Nov 25.
Nanoporous gold (NPG) electrodes were prepared by dealloying sputtered gold:silver alloys. Electrodes of different thicknesses and pore sizes areas were prepared by varying the temperature and duration of the dealloying procedure; these were then used as supports for FAD-dependent glucose dehydrogenase (GDH) (Glomorella cingulata) and bilirubin oxidase (BOx) (Myrothecium verrucaria). Glucose dehydrogenase was immobilized by drop-casting a solution of the enzyme with an osmium redox polymer together with a crosslinked polymer, whereas bilirubin oxidase was attached covalently through carbodiimide coupling to a diazonium-modified NPG electrode. The stability of the bilirubin-oxidase-modified NPG electrode was significantly improved in comparison with that of a planar gold electrode. Enzyme fuel cells were also prepared; the optimal response was obtained with a BOx-modified NPG cathode (500 nm thickness) and a GDH-modified anode (300 nm), which generated power densities of 17.5 and 7.0 μW cm in phosphate-buffered saline and artificial serum, respectively.
通过对溅射的金银合金进行脱合金处理制备了纳米多孔金（NPG）电极。通过改变脱合金过程的温度和持续时间制备了不同厚度和孔径区域的电极；然后将这些电极用作依赖黄素腺嘌呤二核苷酸（FAD）的葡萄糖脱氢酶（GDH）（缢缩小球腔菌）和胆红素氧化酶（BOx）（疣孢漆斑菌）的载体。通过将酶溶液与锇氧化还原聚合物以及交联聚合物一起滴铸来固定葡萄糖脱氢酶，而胆红素氧化酶则通过碳二亚胺偶联共价连接到重氮修饰的NPG电极上。与平面金电极相比，胆红素氧化酶修饰的NPG电极的稳定性得到了显著提高。还制备了酶燃料电池；使用BOx修饰的NPG阴极（厚度500 nm）和GDH修饰的阳极（300 nm）获得了最佳响应，在磷酸盐缓冲盐水和人工血清中分别产生了17.5和7.0 μW cm的功率密度。
