Department of Chemical Sciences and Bernal Institute, University of Limerick , Limerick V94 T9PX, Ireland.
School of Chemistry & Ryan Institute, National University of Ireland Galway , Galway H91 TK33, Ireland.
ACS Appl Mater Interfaces. 2018 Feb 28;10(8):7107-7116. doi: 10.1021/acsami.7b18708. Epub 2018 Feb 13.
A lactate/O enzymatic biofuel cell (EBFC) was prepared as a potential power source for wearable microelectronic devices. Mechanically stable and flexible nanoporous gold (NPG) electrodes were prepared using an electrochemical dealloying method consisting of a pre-anodization process and a subsequent electrochemical cleaning step. Bioanodes were prepared by the electrodeposition of an Os polymer and Pediococcus sp. lactate oxidase onto the NPG electrode. The electrocatalytic response to lactate could be tuned by adjusting the deposition time. Bilirubin oxidase from Myrothecium verrucaria was covalently attached to a diazonium-modified NPG surface. A flexible EBFC was prepared by placing the electrodes between two commercially available contact lenses to avoid direct contact with the eye. When tested in air-equilibrated artificial tear solutions (3 mM lactate), a maximum power density of 1.7 ± 0.1 μW cm and an open-circuit voltage of 380 ± 28 mV were obtained, values slightly lower than those obtained in phosphate buffer solution (2.4 ± 0.2 μW cm and 455 ± 21 mV, respectively). The decrease was mainly attributed to interference from ascorbate. After 5.5 h of operation, the EBFC retained 20% of the initial power output.
一种乳酸/O 酶生物燃料电池（EBFC）被制备为用于可穿戴微电子设备的潜在电源。使用包括预阳极氧化过程和随后的电化学清洗步骤的电化学脱合金方法制备机械稳定且灵活的纳米多孔金（NPG）电极。生物阳极通过将 Os 聚合物和肠膜明串珠菌乳酸氧化酶电沉积到 NPG 电极上制备。通过调整沉积时间可以调整对乳酸的电催化响应。来自疣孢漆斑菌的胆红素氧化酶通过共价键附着到重氮改性的 NPG 表面。通过将电极放置在两个市售隐形眼镜之间来制备柔性 EBFC，以避免与眼睛直接接触。在空气平衡的人工泪液溶液（3 mM 乳酸）中进行测试时，获得了 1.7 ± 0.1 μW cm 的最大功率密度和 380 ± 28 mV 的开路电压，这些值略低于在磷酸盐缓冲溶液中获得的值（分别为 2.4 ± 0.2 μW cm 和 455 ± 21 mV）。这种下降主要归因于抗坏血酸的干扰。在 5.5 小时的运行后，EBFC 保留了初始功率输出的 20%。
