Pombo-García Karina, Rühl Carmen L, Lam Raymond, Barreto José A, Ang Ching-Seng, Scammells Peter J, Comba Peter, Spiccia Leone, Graham Bim, Joshi Tanmaya, Stephan Holger
Institute of Radiopharmaceutical Cancer Research, Helmholtz-Zentrum Dresden-Rossendorf, 01328, Dresden, Germany.
Heidelberg University, Institute of Inorganic Chemistry and Interdisciplinary Centre for Scientific Computing, Im Neuenheimer Feld 270, 69120, Heidelberg, Germany.
Chempluschem. 2017 Apr;82(4):638-646. doi: 10.1002/cplu.201700052. Epub 2017 Mar 27.
Polyacrylic-acid-coated ultra-small super-paramagnetic iron oxide nanoparticles were surface-modified with low-molecular-weight sulfobetaines or 3-(diethylamino)propylamine in order to generate nanoparticles with zwitterionic character (ZW-NPs). The ZW-NPs proved highly resistant to serum protein corona formation in vitro, as revealed by atomic force microscopy, SDS-PAGE and proteomics analysis, and exhibited low cytotoxicity towards A431 and HEK293 cells. The presence of unreacted carboxylic acid groups enabled additional functionalization with fluorescent (Cy5) and radioactive [ Cu-dmptacn; dmptacn=1,4-bis(2-pyridinylmethyl)-1,4,7-triazacyclononane] moieties. Overall, the ZW-NPs represent promising platforms for the development of new multimodal diagnostic/therapeutic agents possessing "stealth" properties.
用低分子量磺基甜菜碱或3-(二乙氨基)丙胺对聚丙烯酸包覆的超小超顺磁性氧化铁纳米颗粒进行表面改性，以制备具有两性离子特性的纳米颗粒(ZW-NPs)。原子力显微镜、SDS-PAGE和蛋白质组学分析表明，ZW-NPs在体外对血清蛋白冠层的形成具有高度抗性，并且对A431和HEK293细胞表现出低细胞毒性。未反应的羧酸基团的存在使得能够用荧光(Cy5)和放射性[Cu-dmptacn；dmptacn = 1,4-双(2-吡啶基甲基)-1,4,7-三氮杂环壬烷]部分进行额外的功能化。总的来说，ZW-NPs是开发具有“隐身”特性的新型多模态诊断/治疗剂的有前景的平台。
