一种使用门控纳米多孔阳极氧化铝的支原体基因组DNA探针。

A Mycoplasma Genomic DNA Probe using Gated Nanoporous Anodic Alumina.

作者信息

Pla Luís, Xifré-Pérez Elisabet, Ribes Àngela, Aznar Elena, Marcos M Dolores, Marsal Lluís F, Martínez-Máñez Ramón, Sancenón Félix

机构信息

Departamento de Química, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022, Valencia, Spain.

Instituto Interuniversitario de Investigación de Reconocimiento Molecular, y Desarrollo Tecnológico (IDM), Universitat Politécnica de Valencia, Universitat de Valencia, Camino de Vera, s/n, 46022, Valencia, Spain.

出版信息

Chempluschem. 2017 Mar;82(3):337-341. doi: 10.1002/cplu.201600651. Epub 2017 Jan 12.

DOI:10.1002/cplu.201600651

PMID:31962016

Abstract

A nanoporous anodic alumina (NAA)-based sensor system for the detection of Mycoplasma was developed through the implementation of "molecular gates" selective to the presence of this bacterium. The capped support showed a negligible cargo release, while presence of Mycoplasma genomic DNA resulted in the release of rhodamine B fluorescent dye. This sensor system presents a limit of detection of 20 genomic DNA copies⋅μL and was applied to the detection of Mycoplasma bacteria in competitive environments, such as culture cell media.

摘要

通过实施对这种细菌存在具有选择性的“分子门”，开发了一种基于纳米多孔阳极氧化铝（NAA）的用于检测支原体的传感器系统。带帽载体显示出可忽略不计的货物释放，而支原体基因组DNA的存在导致罗丹明B荧光染料的释放。该传感器系统的检测限为20个基因组DNA拷贝·μL，并应用于在竞争环境（如培养细胞培养基）中检测支原体细菌。