Garrido-Cano Iris, Pla Luis, Santiago-Felipe Sara, Simón Soraya, Ortega Belen, Bermejo Begoña, Lluch Ana, Cejalvo Juan Miguel, Eroles Pilar, Martínez-Máñez Ramón
Biomedical Research Institute INCLIVA, 46010 Valencia, Spain.
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), 28029 Madrid, Spain.
ACS Sens. 2021 Mar 26;6(3):1022-1029. doi: 10.1021/acssensors.0c02222. Epub 2021 Feb 18.
Circulating microRNAs have emerged as potential diagnostic biomarkers. The deregulation of the microRNA miR-99a-5p has been previously described as an effective biomarker of early breast cancer. Herein, we present a new nanoporous anodic alumina (NAA)-based biosensor that can detect plasma miR-99a-5p with high sensitivity and selectivity. NAA pores are loaded with rhodamine B and capped with a specific oligonucleotide that is able to block cargo release until the target is present. In the presence of miR-99a-5p, the capping oligonucleotide recognizes the miR-99a-5p sequence and displaces it allowing the release of the encapsulated dye. This method is able to successfully distinguish healthy controls from breast cancer patients, even at early stages with high efficiency, showing the presented system as a promising tool for breast cancer detection.
循环微RNA已成为潜在的诊断生物标志物。此前已将微RNA miR-99a-5p的失调描述为早期乳腺癌的有效生物标志物。在此，我们展示了一种基于新型纳米多孔阳极氧化铝（NAA）的生物传感器，它能够高灵敏度和高选择性地检测血浆中的miR-99a-5p。NAA孔中装载有罗丹明B，并由一种特定的寡核苷酸封闭，该寡核苷酸能够阻止货物释放，直到目标物出现。在miR-99a-5p存在的情况下，封闭寡核苷酸识别miR-99a-5p序列并将其置换，从而使封装的染料得以释放。该方法能够成功区分健康对照者和乳腺癌患者，即使在早期阶段也具有高效率，表明所展示的系统是一种有前景的乳腺癌检测工具。
