College of Horticulture/Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, China.
The New Zealand Institute for Plant & Food Research Limited (Plant & Food Research) Mt Albert, Private Bag 92169, Auckland Mail Centre, Auckland 1142, New Zealand.
Int J Biol Macromol. 2020 Apr 1;148:817-832. doi: 10.1016/j.ijbiomac.2020.01.167. Epub 2020 Jan 18.
MYB transcription factors comprise one of the largest families in plant kingdom, which play a variety of functions in plant developmental processes and defence responses, the R2R3-MYB members are the predominant form found in higher plants. In the present study, a total of 111 StR2R3-MYB transcription factors were identified and further phylogenetically classified into 31 subfamilies, as supported by highly conserved gene structures and motifs. Collinearity analysis showed that the segmental duplication events played a crucial role in the expansion of StR2R3-MYB gene family. Synteny analysis indicated that 37 and 13 StR2R3-MYB genes were orthologous to Arabidopsis and wheat (Triticum aestivum), respectively, and these gene pairs have evolved under strong purifying selection. RNA-seq data from different tissues and abiotic stresses revealed tissue-preferential and abiotic stress-responsive StR2R3-MYB genes. We further analyzed StR2R3-MYB genes might be involved in anthocyanin biosynthesis and drought stress by using RNA-seq data of pigmented tetraploid potato cultivars and drought-sensitive and -tolerant tetraploid potato cultivars under drought stress, respectively. Moreover, EAR motifs were found in 21 StR2R3-MYB proteins and 446 pairs of proteins were predicted to interact with 21 EAR motif-containing StR2R3-MYB proteins by constructing the interaction network with medium confidence (0.4). Additionally, Gene Ontology (GO) analysis of the 21 EAR motif-containing StR2R3-MYB proteins was performed to further investigate their functions. This work will facilitate future biologically functional studies of potato StR2R3-MYB transcription factors and enrich the knowledge of MYB superfamily genes in plant species.
MYB 转录因子是植物界最大的家族之一，它们在植物发育过程和防御反应中发挥着多种功能，R2R3-MYB 成员是在高等植物中发现的主要形式。在本研究中，共鉴定出 111 个 StR2R3-MYB 转录因子，并进一步根据高度保守的基因结构和基序进行了系统发育分类，分为 31 个亚家族。共线性分析表明，片段复制事件在 StR2R3-MYB 基因家族的扩张中起关键作用。同线性分析表明，37 个和 13 个 StR2R3-MYB 基因分别与拟南芥和小麦（Triticum aestivum）同源，这些基因对在强烈的纯化选择下进化。来自不同组织和非生物胁迫的 RNA-seq 数据表明，StR2R3-MYB 基因具有组织偏好性和对非生物胁迫的反应性。我们进一步分析了 StR2R3-MYB 基因可能通过使用有色四倍体马铃薯品种和干旱敏感和耐干旱的四倍体马铃薯品种的 RNA-seq 数据参与花青素生物合成和干旱胁迫。此外，在 21 个 StR2R3-MYB 蛋白中发现了 EAR 基序，通过构建具有中等置信度（0.4）的互作网络，预测了 446 对蛋白与包含 21 个 EAR 基序的 StR2R3-MYB 蛋白相互作用。此外，对包含 21 个 EAR 基序的 StR2R3-MYB 蛋白进行了基因本体论（GO）分析，以进一步研究其功能。这项工作将有助于未来对马铃薯 StR2R3-MYB 转录因子的生物学功能研究，并丰富植物物种中 MYB 超家族基因的知识。
