Li Gang, Zhou Hua, He Yuxiang, Sun Shunji, Wu Xuejun, Yuan Hai
Department of Vascular Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
Vascular Intervention Department, Hospital Affiliated to Weifang Medical University, Weifang, China.
J Vasc Res. 2020;57(2):58-64. doi: 10.1159/000504848. Epub 2020 Jan 21.
Aortic mural inflammatory damage takes a vital part in abdominal aortic aneurysm (AAA). Recently, ulinastatin (UTI) has attracted attention for its anti-inflammatory function. Our study aimed to evaluate potential influences of UTI on experimental AAA.
A mouse model of AAA was induced by infusion of porcine pancreatic elastase (PPE) into the abdominal aorta. Mice were treated with UTI (50,000 U/kg/day i.p.) beginning either immediately or on the 4th day after PPE infusion, with treatment being continued until the 14th day. UTI effects were assessed by aortic diameter measurements and aortic histopathological analysis.
Significant and time-dependent aortic diameter enlargement persisted in the control mice from day 0. In the UTI group, aortic diameter increased, and depletion of aortic mural smooth muscle cells and elastin was significantly -attenuated. Simultaneously, mural CD68+ macrophages, CD8+ T-cell and B220+ B-cell density, as well as neoangiogenesis were suppressed by UTI. In addition, delayed UTI treatment could still effectively limit aneurysm expansion.
UTI treatment limits the formation and growth of experimental AAA, and UTI may be a potential treatment for early AAA disease.
主动脉壁炎症损伤在腹主动脉瘤（AAA）中起关键作用。近来，乌司他丁（UTI）因其抗炎功能而受到关注。我们的研究旨在评估UTI对实验性AAA的潜在影响。
通过向腹主动脉内注入猪胰弹性蛋白酶（PPE）诱导建立AAA小鼠模型。小鼠在PPE注入后立即或第4天开始接受UTI治疗（腹腔注射，50,000 U/kg/天），持续治疗至第14天。通过测量主动脉直径和进行主动脉组织病理学分析来评估UTI的作用。
从第0天起，对照小鼠的主动脉直径持续显著且随时间增大。在UTI组中，主动脉直径增加，主动脉壁平滑肌细胞和弹性蛋白的损耗明显减轻。同时，UTI抑制了壁内CD68+巨噬细胞、CD8+ T细胞和B220+ B细胞密度以及新生血管形成。此外，延迟的UTI治疗仍可有效限制动脉瘤扩张。
UTI治疗可限制实验性AAA的形成和生长，UTI可能是早期AAA疾病的一种潜在治疗方法。
