Institute of Cardiovascular Science, Basic Medical School of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, China.
Laboratory Animal Center, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China.
J Immunol Res. 2021 Nov 16;2021:6297332. doi: 10.1155/2021/6297332. eCollection 2021.
Elastase-induced abdominal aortic aneurysm (AAA) model is widely used for aneurysmal pathogenesis and translational research. However, temporal alternations in aneurysmal histologies remain unknown. This study is aimed at analyzing temporal immunopathologies of aneurysmal aorta following experimental AAA induction.
Male C57BL/6J mice at the age of 10-14 weeks received intra-aortic infusion of elastase to induce AAAs. Aortic diameters at the baseline and indicated days after AAA induction were measured, and aortae were collected for histopathological analysis.
Aorta diameters increased from 0.52 mm at the baseline levels to 0.99 mm, 1.34 mm, and 1.41 mm at days 7, 14, and 28, respectively, corresponding 90%, 158%, and 171% increases over the baseline level. Average aortic diameters did not differ between days 14 and 28. Severe elastin degradation and smooth muscle cell depletion were found at days 14 and 28 as compared to the baseline and day 7. No difference in the scores of medial elastin and SMC destruction was noted between days 14 and 28. Consistent results were found for leukocyte accumulation, neoangiogenesis, and matrix metalloproteinase expression. Twenty-eight days after AAA induction, all aneurysmal pathologies showed an attenuated trend, although most histopathological parameters did no differ between days 14 and 28.
Our data suggest that almost aneurysmal immunohistopathologies reach maximal 14 days following AAA induction. Analysis of day 14 histologies is sufficient for AAA pathogenesis and translational studies in elastase-induced mouse experimental AAAs.
弹性蛋白酶诱导的腹主动脉瘤（AAA）模型广泛用于动脉瘤发病机制和转化研究。然而，动脉瘤组织学的时间变化尚不清楚。本研究旨在分析实验性 AAA 诱导后动脉瘤主动脉的时间性免疫病理学。
10-14 周龄雄性 C57BL/6J 小鼠接受主动脉内弹性蛋白酶输注以诱导 AAA。在 AAA 诱导后的指定天数测量主动脉直径，并收集主动脉进行组织病理学分析。
主动脉直径从基线水平的 0.52mm 增加到第 7、14 和 28 天的 0.99mm、1.34mm 和 1.41mm，分别比基线水平增加了 90%、158%和 171%。第 14 天和第 28 天的平均主动脉直径之间没有差异。与基线和第 7 天相比，第 14 天和第 28 天发现严重的弹性蛋白降解和平滑肌细胞耗竭。中膜弹性蛋白和 SMC 破坏评分在第 14 天和第 28 天之间没有差异。白细胞积累、新生血管形成和基质金属蛋白酶表达的结果一致。在 AAA 诱导后 28 天，所有动脉瘤病理均呈减弱趋势，尽管第 14 天和第 28 天之间的大多数组织病理学参数没有差异。
我们的数据表明，在 AAA 诱导后 14 天内几乎所有的动脉瘤免疫组织病理学都达到了最大值。分析第 14 天的组织学对于弹性蛋白酶诱导的小鼠实验性 AAA 中的 AAA 发病机制和转化研究是足够的。
