Fan Rongbo, Xie Shubin, Wang Shifeng, Yu Zhongna, Sun Xueheng, Du Qijing, Yang Yongxin, Han Rongwei
College of Food Science and Engineering, Qingdao Agricultural University, Qingdao 266109, Shandong, China.
Haidu College. Qingdao Agricultural University, Laiyang 265200, Shandong, China.
Food Chem X. 2023 Feb 16;17:100601. doi: 10.1016/j.fochx.2023.100601. eCollection 2023 Mar 30.
This study investigated the differences in proteins and metabolites from goat and bovine milk, and their mixtures, using data-independent-acquisition-based proteomics and metabolomics methods. In the skim milk, relative abundances of secretoglobin family 1D member (SCGB1D), polymeric immunoglobulin receptor, and glycosylation-dependent cell adhesion molecule 1 were increased, with an increase in the amount of 1-100 % bovine milk and served as markers at the 1 % adulteration level. In whey samples, β-lactoglobulin and α-2-HS-glycoprotein could be used to detect adulteration at the 0.1 % adulteration level, and SCGB1D and zinc-alpha-2-glycoprotein at the 1 % level. The metabolites of uric acid and -formylkynurenine could be used to detect bovine milk at adulteration levels as low as 1 % based on variable importance at a projection value of > 1.0 and P-value of < 0.05. Our findings suggest novel markers of SCGB1D, uric acid, and -formylkynurenine that can help to facilitate assessments of goat milk authenticity.
本研究采用基于数据非依赖采集的蛋白质组学和代谢组学方法，研究了山羊奶、牛奶及其混合物中蛋白质和代谢物的差异。在脱脂奶中，随着牛奶含量从1%增加到100%，分泌球蛋白家族1D成员（SCGB1D）、聚合免疫球蛋白受体和糖基化依赖性细胞黏附分子1的相对丰度增加，并作为1%掺假水平的标志物。在乳清样品中，β-乳球蛋白和α-2-HS-糖蛋白可用于检测0.1%掺假水平的掺假情况，而SCGB1D和锌-α-2-糖蛋白可用于检测1%水平的掺假情况。基于投影值>1.0和P值<0.05时的变量重要性，尿酸和N-甲酰犬尿氨酸的代谢物可用于检测低至1%掺假水平的牛奶。我们的研究结果表明，SCGB1D、尿酸和N-甲酰犬尿氨酸是新的标志物，有助于促进对山羊奶真实性的评估。
Zotero 插件
