通过胰蛋白酶鉴定从人血浆和血浆蛋白科恩IV-4组分中释放的[羟脯氨酸3]-缓激肽。

Identification of [hydroxyproline3]-bradykinin released from human plasma and plasma protein Cohn's fraction IV-4 by trypsin.

作者信息

Sasaguri M, Ikeda M, Ideishi M, Arakawa K

机构信息

Department of Internal Medicine, Fukuoka University, School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1988 Nov 30;157(1):210-7. doi: 10.1016/s0006-291x(88)80034-2.

DOI:10.1016/s0006-291x(88)80034-2

PMID:3196332

Abstract

Aside from bradykinin (BK), a novel kinin, [Hydroxyproline3]-bradykinin ( [Hyp3]-BK), was isolated from the reaction mixture of human plasma and plasma protein Cohn's fraction IV-4 with trypsin. The liberated kinins were isolated based on procedures which we previously described for the isolation of [Hyp3]-lysyl-bradykinin ( [Hyp3]-Lys-BK) formed by kallikrein. The ratio of the amounts of two kinins thus formed from human plasma protein Cohn's fraction IV-4 were [Hyp3]-BK 25 +/- 4% and BK 75 +/- 4%, similarly to that of [Hyp3]-Lys-BK and Lys-BK, formed by kallikrein, but it varied by persons. The isolation of [Hyp3]-BK and [Hyp3]-Lys-BK suggests that a novel kininogen containing hydroxyproline in the third position of the bradykinin sequence in human plasma protein, possibly undergone post-translational modifications.

摘要

除了缓激肽（BK）外，一种新型激肽，[羟脯氨酸3] - 缓激肽（[Hyp3] - BK），从人血浆与血浆蛋白科恩IV - 4组分和胰蛋白酶的反应混合物中分离出来。释放的激肽是根据我们之前描述的用于分离由激肽释放酶形成的[Hyp3] - 赖氨酰缓激肽（[Hyp3] - Lys - BK）的程序分离的。从人血浆蛋白科恩IV - 4组分中如此形成的两种激肽的量的比例为[Hyp3] - BK 25±4%和BK 75±4%，类似于由激肽释放酶形成的[Hyp3] - Lys - BK和赖氨酰缓激肽（Lys - BK）的比例，但因人而异。[Hyp3] - BK和[Hyp3] - Lys - BK的分离表明，人血浆蛋白中缓激肽序列第三位含有羟脯氨酸的一种新型激肽原，可能经历了翻译后修饰。