Tensen C P, Cox K J, Smit A B, van der Schors R C, Meyerhof W, Richter D, Planta R J, Hermann P M, van Minnen J, Geraerts W P, Knol J C, Burke J F, Vreugdenhil E, van Heerikhuizen H
Department of Biochemistry and Molecular Biology, Research Institute Neurosciences, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands.
J Neurosci. 1998 Dec 1;18(23):9812-21. doi: 10.1523/JNEUROSCI.18-23-09812.1998.
A novel G-protein-coupled receptor (GRL106) resembling neuropeptide Y and tachykinin receptors was cloned from the mollusc Lymnaea stagnalis. Application of a peptide extract from the Lymnaea brain to Xenopus oocytes expressing GRL106 activated a calcium-dependent chloride channel. Using this response as a bioassay, we purified the ligand for GRL106, Lymnaea cardioexcitatory peptide (LyCEP), an RFamide-type decapeptide (TPHWRPQGRF-NH2) displaying significant similarity to the Achatina cardioexcitatory peptide (ACEP-1) as well as to the recently identified family of mammalian prolactin-releasing peptides. In the Lymnaea brain, the cells that produce egg-laying hormone are the predominant site of GRL106 gene expression and appear to be innervated by LyCEP-containing fibers. Indeed, LyCEP application transiently hyperpolarizes isolated egg-laying hormone cells. In the Lymnaea pericardium, LyCEP-containing fibers end blindly at the pericardial lumen, and the heart is stimulated by LyCEP in vitro. These data confirm that LyCEP is an RFamide ligand for GRL106.
从椎实螺（Lymnaea stagnalis）中克隆出一种类似神经肽Y和速激肽受体的新型G蛋白偶联受体（GRL106）。将来自椎实螺脑的肽提取物应用于表达GRL106的非洲爪蟾卵母细胞，可激活一种钙依赖性氯离子通道。利用这一反应作为生物测定法，我们纯化了GRL106的配体——椎实螺心脏兴奋肽（LyCEP），它是一种RF酰胺型十肽（TPHWRPQGRF-NH2），与玛瑙螺心脏兴奋肽（ACEP-1）以及最近鉴定出的哺乳动物催乳素释放肽家族具有显著相似性。在椎实螺脑中，产生产卵激素的细胞是GRL106基因表达的主要部位，并且似乎受含LyCEP的纤维支配。实际上，应用LyCEP可使分离出的产卵激素细胞短暂超极化。在椎实螺心包中，含LyCEP的纤维在心包腔内盲端终止，并且在体外LyCEP可刺激心脏。这些数据证实LyCEP是GRL106的一种RF酰胺配体。
