Department of Biology, Faculty of Science, Mahidol University, Ratchathewi, Bangkok 10400, Thailand.
Snake Farm, Queen Saovabha Memorial Institute, The Thai Red Cross Society, Pathumwan, Bangkok 10330, Thailand.
Toxins (Basel). 2020 Jan 16;12(1):54. doi: 10.3390/toxins12010054.
The two venomous pit vipers, and , are distributed throughout Thailand, although their abundance varies among different areas. No species-specific antivenom is available for their bite victims, and the only recorded treatment method is a horse antivenom raised against crude venom. To facilitate assessment of the cross-reactivity of heterologous antivenoms, protein profiles of and venoms were explored using mass-spectrometry-based proteomics. The results show that 185 and 216 proteins were identified from and venoms, respectively. Two major protein components in and venoms were snake venom serine protease and metalloproteinase. The toxicity of the venoms on human monocytes and skin fibroblasts was analyzed, and both showed a greater cytotoxic effect on fibroblasts than monocytic cells, with toxicity occurring in a dose-dependent rather than a time-dependent manner. Exploring the protein composition of snake venom leads to a better understanding of the envenoming of prey. Moreover, knowledge of pit viper venomics facilitates the selection of the optimum heterologous antivenoms for treating bite victims.
这两种毒蛇， 和 ，分布于整个泰国，尽管它们在不同地区的数量有所不同。针对它们咬伤的受害者，没有特定于物种的抗蛇毒血清，唯一记录的治疗方法是针对 粗毒液制备的马抗蛇毒血清。为了方便评估异种抗蛇毒血清的交叉反应性，使用基于质谱的蛋白质组学方法研究了 和 毒液的蛋白质图谱。结果表明，从 和 毒液中分别鉴定出 185 和 216 种蛋白质。和 毒液中的两种主要蛋白质成分是蛇毒丝氨酸蛋白酶和金属蛋白酶。分析了毒液对人单核细胞和皮肤成纤维细胞的毒性，结果表明两种毒液对成纤维细胞的细胞毒性均大于单核细胞，且毒性呈剂量依赖性而非时间依赖性。探索蛇毒的蛋白质组成有助于更好地了解猎物的中毒情况。此外，了解响尾蛇毒液组学有助于选择治疗咬伤受害者的最佳异种抗蛇毒血清。
