Dziedziech Alexis, Shivankar Sai, Theopold Ulrich
Department of Molecular Biosciences, The Wenner-Gren Institute (MBW), Stockholm University, 11419 Stockholm, Sweden.
Insects. 2020 Jan 18;11(1):60. doi: 10.3390/insects11010060.
Entomopathogenic nematodes (EPNs) have been a useful model for studying wound healing in insects due to their natural mechanism of entering an insect host either through the cuticle or an orifice. While many experiments have shed light on nematode and host behavior, as well as the host immune response, details regarding early nematode entry and proliferative events have been limited. Using high-resolution microscopy, we provide data on the early infection kinetics of and its symbiotic bacteria, . EPNs appendage themselves to the host and enter through the host cuticle with a drill-like mechanism while leaving their outer sheath behind. EPNs immediately release their symbiotic bacteria in the host which leads to changes in host behavior and septicemia within 6 h while EPNs travel through the host in a predictable manner, congregating in the anterior end of the host. This paper sheds light on the entry and proliferative events of EPN infection, which will further aid in our understanding of wound healing and host immune activation at a high spatiotemporal resolution.
昆虫病原线虫（EPNs）因其通过表皮或孔口进入昆虫宿主的天然机制，成为研究昆虫伤口愈合的有用模型。虽然许多实验揭示了线虫和宿主的行为以及宿主免疫反应，但关于线虫早期进入和增殖事件的细节却很有限。利用高分辨率显微镜，我们提供了关于 及其共生细菌 的早期感染动力学数据。EPNs附着在宿主身上，通过类似钻孔的机制穿过宿主表皮，同时留下其外层鞘。EPNs立即在宿主体内释放其共生细菌，这会导致宿主行为改变和6小时内发生败血症，而EPNs以可预测的方式在宿主体内移动，聚集在宿主前端。本文揭示了EPN感染的进入和增殖事件，这将进一步有助于我们在高时空分辨率下理解伤口愈合和宿主免疫激活。
