Kunc Martin, Arefin Badrul, Hyrsl Pavel, Theopold Ulrich
a Department of Molecular Biosciences, The Wenner-Gren Institute (MBW) , Stockholm University , Stockholm , Sweden.
b Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science , Masaryk University , Brno , Czech Republic.
Fly (Austin). 2017 Jul 3;11(3):208-217. doi: 10.1080/19336934.2017.1297350. Epub 2017 Feb 21.
One of the key factors that determine the interaction between hosts and their parasites is the frequency of their interactions, which depends on the locomotory behavior of both parts. To address host behavior we used natural infections involving insect pathogenic nematodes and Drosophila melanogaster larvae as hosts. Using a modified version of a recently described method (FIMTrack) to assess several parameters in larger sets of animals, we initially detected specific differences in larval food searching when comparing Drosophila strains. These differences were further influenced by the presence of nematodes. Given a choice, Drosophila larvae clearly avoided nematodes irrespective of their genetic background. Our newly developed methods will be useful to test candidate genes and pathways involved in host/pathogen interactions in general and to assess specific parameters of their interaction.
决定宿主与其寄生虫之间相互作用的关键因素之一是它们相互作用的频率，这取决于双方的运动行为。为了研究宿主行为，我们利用涉及昆虫病原线虫和黑腹果蝇幼虫作为宿主的自然感染。通过使用最近描述的一种方法（FIMTrack）的改进版本来评估大量动物的几个参数，我们最初在比较果蝇品系时检测到幼虫食物搜索的特定差异。这些差异进一步受到线虫存在的影响。在有选择的情况下，果蝇幼虫明显避开线虫，无论其遗传背景如何。我们新开发的方法将有助于一般地测试参与宿主/病原体相互作用的候选基因和途径，并评估它们相互作用的特定参数。
