State Key Laboratory of Tea Plant Biology and Utilization, International Joint Laboratory on Tea Chemistry and Health Effects, Anhui Agricultural University, Hefei 230036, China.
Int J Mol Sci. 2020 Jan 19;21(2):655. doi: 10.3390/ijms21020655.
Terpenoids play vital roles in tea aroma quality and plants defense performance determination, whereas the scenarios of genes to metabolites of terpenes pathway remain uninvestigated in tea plants. Here, we report the use of an integrated approach combining metabolites, target gene transcripts and function analyses to reveal a gene-to-terpene network in tea plants. Forty-one terpenes including 26 monoterpenes, 14 sesquiterpenes and one triterpene were detected and 82 terpenes related genes were identified from five tissues of tea plants. Pearson correlation analysis resulted in genes to metabolites network. One terpene synthases whose expression positively correlated with farnesene were selected and its function was confirmed involved in the biosynthesis of -farnesene, -ocimene and -farnesene, a very important and conserved alarm pheromone in response to aphids by both in vitro enzymatic assay in planta function analysis. In summary, we provided the first reliable gene-to-terpene network for novel genes discovery.
萜类化合物在茶叶香气质量和植物防御性能的决定中起着至关重要的作用，而萜类化合物的基因到代谢物途径的情况在茶树中尚未得到研究。在这里，我们报告了一种综合方法的应用，该方法结合了代谢物、靶基因转录本和功能分析，以揭示茶树中的基因萜类网络。从茶树的五个组织中检测到 41 种萜类化合物，包括 26 种单萜、14 种倍半萜和 1 种三萜，鉴定出 82 种萜类相关基因。皮尔逊相关分析产生了基因到代谢物网络。选择了一个萜类合酶，其表达与法呢烯呈正相关，并通过体外酶促测定和植物功能分析证实其功能涉及 -法呢烯、-罗勒烯和 -法呢烯的生物合成， -法呢烯、-罗勒烯和 -法呢烯是对蚜虫反应的一种非常重要和保守的报警信息素。综上所述，我们为新基因的发现提供了第一个可靠的基因萜类网络。
