Li Rui, Wang Xia, Zhao Xiaoqian, Zhang Xiaohong, Chen Honghai, Ma Yue, Liu Yandong
Department of Clinical Laboratory, Shenyang Fifth People's Hospital, Shenyang, Liaoning 110021, P.R. China.
Admin Office, Shenyang Fifth People's Hospital, Shenyang, Liaoning 110021, P.R. China.
Oncol Lett. 2020 Feb;19(2):1368-1374. doi: 10.3892/ol.2019.11232. Epub 2019 Dec 20.
Non-small cell lung cancer (NSCLC) is the most common histological type of lung cancer. Altered expression of centromere protein F (CENPF), a transient kinetochore protein, has been found in a variety of human cancers. However, its clinical significance in NSCLC remains unknown. In the present study the results of quantitative PCR and western blot analyses demonstrated that CENPF and Forkhead box M1 (FOXM1) were significantly higher in NSCLC tissues than in the non-cancerous controls at both transcriptional and translational levels. Immunohistochemical staining results showed 58.7% (44/75) and 64.0% (48/75) of NSCLC tissues displayed high expression of CENPF and FOXM1, respectively. CENPF protein expression showed a positive correlation with tumor size (P=0.0179), vital status (P=0.0008) and FOXM1 expression (P=0.0013) in NSCLC. Poor overall survival was correlated with high levels of CENPF and FOXM1 in NSCLC patients as evaluated by Kaplan-Meier and log rank test. Multivariate analyses showed that CENPF expression was an independent prognostic factor for NSCLC. In conclusion, our study provides evidence of the prognostic function of CENPF in NSCLC.
非小细胞肺癌（NSCLC）是肺癌最常见的组织学类型。着丝粒蛋白F（CENPF）是一种瞬时动粒蛋白，其表达改变已在多种人类癌症中被发现。然而，其在NSCLC中的临床意义仍不清楚。在本研究中，定量PCR和蛋白质印迹分析结果表明，在转录和翻译水平上，NSCLC组织中的CENPF和叉头框M1（FOXM1）均显著高于非癌对照。免疫组织化学染色结果显示，分别有58.7%（44/75）和64.0%（48/75）的NSCLC组织表现出CENPF和FOXM1的高表达。在NSCLC中，CENPF蛋白表达与肿瘤大小（P=0.0179）、生存状态（P=0.0008）和FOXM1表达（P=0.0013）呈正相关。通过Kaplan-Meier和对数秩检验评估，NSCLC患者的总生存期较差与CENPF和FOXM1的高水平相关。多变量分析表明，CENPF表达是NSCLC的一个独立预后因素。总之，我们的研究提供了CENPF在NSCLC中预后功能的证据。
