Han Yong, Xu Shujian, Cheng Kai, Diao Caimei, Liu Song, Zou Weiwei, Bi Yueyang
Department of Breast and Thyroid Surgery, Binzhou Medical University Hospital, Binzhou, Shandong 256603, P.R. China.
Department of Health Management, The People's Hospital of South District of Qingdao, Qingdao, Shandong 266000, P.R. China.
Exp Ther Med. 2021 Apr;21(4):401. doi: 10.3892/etm.2021.9832. Epub 2021 Feb 24.
Papillary thyroid cancer (PTHCA) accounts for ~85% cases of thyroid cancer and exhibits high incidence. Targeted therapy is an effective method to combat this disease; however, novel therapeutic targets are required. Centromere protein F (CENPF), a member of centromere proteins and a transient kinetochore protein, regulates various cellular processes such as cell migration and mitosis, and its upregulation has been observed in multiple types of cancer, including breast cancer and gastric cancer. However, the potential role of CENPF in PTHCA progression is remains unclear. The results of the current study demonstrated that CENPF expression was enhanced in human PTHCA tissues through IHC assays. Furthermore, the expression of CENPF was correlated with the prognosis and the clinicopathological features, including T stage (P=0.021) and intraglandular dissemination (P=0.042) in patients with PTHCA. CENPF regulated the proliferation, apoptosis and cell cycle of PTHCA cells , which was confirmed through colony formation, MTT and flow cytometry assays, and affected tumor growth in mice. In conclusion, the current study reported the involvement of CENPF in PTHCA progression and provided a promising therapeutic target for PTHCA treatment.
甲状腺乳头状癌（PTHCA）占甲状腺癌病例的约85%，发病率较高。靶向治疗是对抗这种疾病的有效方法；然而，需要新的治疗靶点。着丝粒蛋白F（CENPF）是着丝粒蛋白的成员之一，也是一种瞬时动粒蛋白，它调节各种细胞过程，如细胞迁移和有丝分裂，并且在包括乳腺癌和胃癌在内的多种癌症中都观察到其表达上调。然而，CENPF在PTHCA进展中的潜在作用仍不清楚。本研究结果通过免疫组化分析表明，CENPF在人PTHCA组织中的表达增强。此外，CENPF的表达与PTHCA患者的预后及临床病理特征相关，包括T分期（P = 0.021）和腺内播散（P = 0.042）。通过集落形成、MTT和流式细胞术分析证实，CENPF调节PTHCA细胞的增殖、凋亡和细胞周期，并影响小鼠肿瘤生长。总之，本研究报道了CENPF参与PTHCA进展，并为PTHCA治疗提供了一个有前景的治疗靶点。
