Wang Yucheng, Chen Kangwu, Chen Hao, Zhang Kai, Lu Jian, Mao Haiqing, Yang Huilin
Department of Orthopedic Surgery, The First Affiliated Hospital of Soochow University Suzhou, Jiangsu, P. R. China.
Int J Clin Exp Pathol. 2017 Nov 1;10(11):10934-10940. eCollection 2017.
Chordoma is a rare, locally aggressive neoplasm of bone, usually with poor prognosis. The treatment for chordoma has been unsatisfactory for decades. MiRNAs were recently introduced into this field and provided new insights to the pathogenesis and pathophysiology of chordoma. However, molecular basis of chordoma remains ambiguous up to now. This research aims to discover novel miRNA molecules as potential biomarkers and therapeutic targets. We measured the expression of miRNA-1290 in chordoma tissues and fetal nucleus pulposus tissues by quantitative real-time PCR. Further, we analyzed its association with the clinical features as well as the prognosis of patients. The expression of miRNA-1290 in chordoma samples was significantly lower than fetal nucleus pulposus samples (P=0.026). Low expression of miRNA-1290 contributed to tumor invasion into surrounding muscle (P=0.013), while no obvious significance was identified between miRNA-1290 expression and patients' age, gender, tumor location and size (P>0.05). Log-rank test showed that low-level miRNA-1290 expression had a prominent impact on the patients' RFS (P=0.004). Conclusively, miRNA-1290 might be a valuable prognostic biomarker and efficient therapeutic target for sacral chordoma.
脊索瘤是一种罕见的、具有局部侵袭性的骨肿瘤，通常预后较差。几十年来，脊索瘤的治疗效果一直不尽人意。微小RNA（miRNA）最近被引入该领域，并为脊索瘤的发病机制和病理生理学提供了新的见解。然而，迄今为止，脊索瘤的分子基础仍不明确。本研究旨在发现新的miRNA分子作为潜在的生物标志物和治疗靶点。我们通过定量实时PCR检测了脊索瘤组织和胎儿髓核组织中miRNA-1290的表达。此外，我们分析了其与临床特征以及患者预后的关系。miRNA-1290在脊索瘤样本中的表达明显低于胎儿髓核样本（P=0.026）。miRNA-1290的低表达促使肿瘤侵袭周围肌肉（P=0.013），而miRNA-1290表达与患者年龄、性别、肿瘤位置和大小之间未发现明显关联（P>0.05）。对数秩检验表明，miRNA-1290低水平表达对患者的无复发生存期（RFS）有显著影响（P=0.004）。总之，miRNA-1290可能是骶骨脊索瘤有价值的预后生物标志物和有效的治疗靶点。
