前列腺癌组织中糖酵解酶表达增加及其与 Gleason 评分的关系。
Increased expression of glycolytic enzymes in prostate cancer tissues and association with Gleason scores.
作者信息
Wang Jun, Li Jitong, Li Xiaojuan, Peng Shubin, Li Jun, Yan Weixin, Cui Yubin, Xiao Hengjun, Wen Xingqiao
机构信息
Department of Urology, The Third Affiliated Hospital of Sun Yat-sen University Guangzhou, P. R. China.
The First Clinical Medicine College, Southern Medical University Guangzhou, P. R. China.
出版信息
Int J Clin Exp Pathol. 2017 Nov 1;10(11):11080-11089. eCollection 2017.
OBJECTIVE
Recent studies have shown that understanding the differences between Gleason 3+4 and Gleason 4+3 in PCa patients may improve their treatment. This study aimed to evaluate the different expression levels of glycolytic proteins for Gleason score of 4+3 and 3+4.
METHODS
A total of 90 PCa patients, including 38 cases with a Gleason score of 7, were included in this study. The expression of glycolytic proteins in both prostate cancer and normal prostate tissues, in GGG2 and GGG3 as well were assessed by immunohistochemical staining.
RESULTS
Compared with GGG3, the GGG2 cases displayed significantly lower expression of all proteins ( < 0.05). The correlation among all enzymes showed that the key glycolytic enzyme, HK2, was significantly positively related to another key enzyme, PKM2 ( = 0.550, < 0.01), and the expression of PFKFB4 was correlated with the expression of HK2 ( = 0.236, < 0.05) and PKM2 ( = 0.392, < 0.01). Additionally, neither GLUT1 nor PFKFB3 was correlated with PFKFB4, HK2 or PKM2. Further analysis showed that HK2 ( = 0.297, < 0.01) and PKM2 ( = 0.431, < 0.01) were significantly positively related to the Gleason score in PCa tissues.
CONCLUSIONS
Glycolytic proteins expression levels were upregulated in PCa tissues. Furthermore, GGG3 exhibits a higher level of glycolysis compared with GGG2 in PCa tissues. Additionally, the key glycolytic enzymes, HK2 and PKM2, are overexpressed simultaneously in PCa and significantly correlate with PCa progression as represented by the GS.
目的
近期研究表明，了解前列腺癌（PCa）患者中Gleason 3+4和Gleason 4+3之间的差异可能会改善其治疗效果。本研究旨在评估Gleason评分为4+3和3+4时糖酵解蛋白的不同表达水平。
方法
本研究共纳入90例PCa患者，其中38例Gleason评分为7分。通过免疫组织化学染色评估前列腺癌组织和正常前列腺组织中糖酵解蛋白的表达，以及在GGG2和GGG3中的表达情况。
结果
与GGG3相比，GGG2病例中所有蛋白的表达均显著降低（<0.05）。所有酶之间的相关性表明，关键糖酵解酶HK2与另一种关键酶PKM2显著正相关（=0.550，<0.01），并且PFKFB4的表达与HK2的表达相关（=0.236，<0.05）以及与PKM2的表达相关（=0.392，<0.01）。此外，GLUT1和PFKFB3均与PFKFB4、HK2或PKM2无相关性。进一步分析表明，HK2（=0.297，<0.01）和PKM2（=0.431，<0.01）与PCa组织中的Gleason评分显著正相关。
结论
PCa组织中糖酵解蛋白表达水平上调。此外，在PCa组织中，与GGG2相比，GGG3表现出更高水平的糖酵解。此外，关键糖酵解酶HK2和PKM2在PCa中同时过度表达，并且与以Gleason评分（GS）表示的PCa进展显著相关。
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