长链非编码 RNA SNHG4 在肝癌中的预后价值及其潜在机制。
The prognostic value of lncRNA SNHG4 and its potential mechanism in liver cancer.
机构信息
Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, P.R. China.
出版信息
Biosci Rep. 2020 Jan 31;40(1). doi: 10.1042/BSR20190729.
BACKGROUND AND OBJECT
Emerging evidence shows that non-coding RNA functions as new gene regulators and prognostic markers in several cancers, including liver cancer. Here, we focused on the small nucleolar RNA host gene 4 (SNHG4) in liver cancer prognosis based on The Cancer Genome Atlas (TCGA) data.
METHODS
The expression data and clinical information were downloaded from TCGA. Chi-square tests evaluated the correlation between SNHG4 expression and clinical parameters. Differences in survival between high and low expression groups (optic cutoff value determined by ROC) from Cox regression analysis were compared, and P-value was calculated by a log-rank test. Kaplan-Meier curves were compared with the log-rank test. GSEA and ceRNA network were conducted to explore the potential mechanism.
RESULTS
Data mining of lncRNA expression data for 371 patients with primary tumor revealed overexpression of SNHG4 in liver cancer. High SNHG4 expression was correlated with histological type (P = 0.01), histologic grade (P = 0.001), stage (P = 0.01), T classification (P = 0.004) and survival status (P = 0.013). Patients with high SNHG4 expression had poor overall survival and relapse-free survival compared with those with low SNHG4 expression. Multivariate analysis identified SNHG4 as an independent prognostic factor of poor survival in liver cancer. GSEA revealed related signaling pathway and ceRNA network explored the further mechanism.
CONCLUSION
High SNHG4 expression is an independent predictor of poor prognosis in liver cancer.
背景与目的
越来越多的证据表明，非编码 RNA 可作为几种癌症（包括肝癌）中新的基因调控因子和预后标志物。在这里，我们基于癌症基因组图谱（TCGA）的数据，主要关注肝癌预后的小核仁 RNA 宿主基因 4（SNHG4）。
方法
从 TCGA 下载表达数据和临床信息。卡方检验评估 SNHG4 表达与临床参数之间的相关性。Cox 回归分析比较高低表达组之间生存差异（通过 ROC 确定光学截止值），并通过对数秩检验计算 P 值。Kaplan-Meier 曲线通过对数秩检验进行比较。进行 GSEA 和 ceRNA 网络分析以探索潜在机制。
结果
对 371 例原发性肿瘤的 lncRNA 表达数据进行数据挖掘，结果显示 SNHG4 在肝癌中呈过表达。高 SNHG4 表达与组织学类型（P=0.01）、组织学分级（P=0.001）、分期（P=0.01）、T 分类（P=0.004）和生存状态（P=0.013）相关。与低 SNHG4 表达的患者相比，高 SNHG4 表达的患者总体生存率和无复发生存率较差。多变量分析确定 SNHG4 是肝癌不良生存的独立预后因素。GSEA 显示相关信号通路，ceRNA 网络进一步探讨了潜在机制。
结论
高 SNHG4 表达是肝癌预后不良的独立预测因子。
Zotero 插件