Department of Hepatobiliary and Pancreatic Surgery, The First Hospital of Jilin University, Changchun, 130021, Jilin, People's Republic of China.
Department of Pathophysiology, College of Basic Medical Sciences, Jilin University, Changchun, 130021, Jilin, People's Republic of China.
Dig Dis Sci. 2019 Jan;64(1):137-143. doi: 10.1007/s10620-018-5315-x. Epub 2018 Oct 4.
Trophinin-associated protein (TROAP) is a cytoplasmic protein that functions as an adhesion molecule in processes such as embryo implantation, spindle formation, and cancer.
To evaluate the relationship of TROAP expression in hepatocellular carcinoma (HCC) tissue with clinicopathologic parameters and survival time in liver cancer patients based on an analysis of The Cancer Genome Atlas Liver Hepatocellular Carcinoma (TCGA-LIHC) data.
RNA-sequencing (RNA-Seq) expression data and clinical information were downloaded for the TCGA-LIHC cohort. Associations between TROAP expression in HCC tissues and clinical parameters were evaluated by Chi-square tests. Differences in survival between high and low expression groups (median expression cutoff) from Cox regression analysis were compared, and P values were calculated by a log-rank test. Kaplan-Meier curves were compared with the log-rank test.
Analysis of RNA-Seq gene expression data for 373 patients with primary tumors revealed overexpression of TROAP in liver cancer. High TROAP expression was associated with survival status (P = 0.015), T stage (P = 0.049), clinical stage (P = 0.048), and gender (P = 0.033). Patients with high TROAP-expressing liver cancers had a shorter median overall survival of 3.83 years compared with 5.80 years for patients with low TROAP-expressing liver cancers (P = 0.00422). Multivariate analysis identified TROAP expression as an independent prognostic variable for overall survival in liver cancer patients.
TROAP expression is an independent predictor of poor survival in liver cancer.
胎球蛋白相关蛋白（TROAP）是一种细胞质蛋白，在胚胎着床、纺锤体形成和癌症等过程中作为黏附分子发挥作用。
根据癌症基因组图谱肝脏肝癌（TCGA-LIHC）数据的分析，评估 TROAP 在肝癌组织中的表达与肝癌患者临床病理参数和生存时间的关系。
下载 TCGA-LIHC 队列的 RNA 测序（RNA-Seq）表达数据和临床信息。通过卡方检验评估 HCC 组织中 TROAP 表达与临床参数之间的相关性。通过 Cox 回归分析比较高低表达组（中位表达截止值）之间的生存差异，并通过对数秩检验计算 P 值。通过 Kaplan-Meier 曲线比较对数秩检验。
对 373 例原发性肿瘤患者的 RNA-Seq 基因表达数据进行分析，发现肝癌中 TROAP 表达上调。高 TROAP 表达与生存状态（P=0.015）、T 分期（P=0.049）、临床分期（P=0.048）和性别（P=0.033）相关。高 TROAP 表达的肝癌患者中位总生存期为 3.83 年，而低 TROAP 表达的肝癌患者中位总生存期为 5.80 年（P=0.00422）。多变量分析确定 TROAP 表达是肝癌患者总生存的独立预后变量。
TROAP 表达是肝癌患者不良生存的独立预测因子。
