Chen Wei, Du Jingjie, Li Xiaodi, Zhi Ziming, Jiang Songshan
Department of Gynecology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, PR China.
Department of Biological Sciences & Technology, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China.
Pharmacogenomics. 2020 Feb;21(3):195-207. doi: 10.2217/pgs-2019-0122.
miR-137 is downregulated in various cancers; however, its function in ovarian cancer remains unclear. The roles of miR-137 in apoptosis were accessed through IC values and DAPI assay. The regulation of MCL1 by miR-137 was investigated through luciferase reporter assay and immunoblot. miR-137 mimic could decrease the IC value of cisplatin and promote apoptosis in OVCAR3 ovarian cancer cells. Using luciferase assay, results on a panel of anti-apoptotic proteins, we identified MCL1 as a target for miR-137 and the results were confirmed using immunoblot. Finally, the underlying pathway in which miR-137 may be involved was investigated by transcriptome sequencing. These results suggest that miR-137 downregulates MCL1 in ovarian cancer cells and mediates cisplatin-induced apoptosis.
miR-137在多种癌症中表达下调；然而，其在卵巢癌中的功能仍不清楚。通过IC值和DAPI检测评估miR-137在细胞凋亡中的作用。通过荧光素酶报告基因检测和免疫印迹研究miR-137对MCL1的调控。miR-137模拟物可降低顺铂对OVCAR3卵巢癌细胞的IC值并促进其凋亡。通过荧光素酶检测一组抗凋亡蛋白，我们确定MCL1是miR-137的靶标，免疫印迹结果证实了这一点。最后，通过转录组测序研究了miR-137可能参与的潜在途径。这些结果表明，miR-137在卵巢癌细胞中下调MCL1并介导顺铂诱导的凋亡。
