Abasaheb Garware College, Pune, Maharashtra 411004, India.
National Centre for Microbial Resource, National Centre for Cell Science, Pune, Maharashtra 411021, India.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1876-1881. doi: 10.1099/ijsem.0.003986.
A haloarchaeal strain (ESP3B_9) was isolated from the salt pan of Sambhar salt lake, Rajasthan, India. Cells were coccoid, non-motile, Gram-stain-negative and formed reddish-pink pigmented colonies. The strain was aerobic, able to grow at 35-55 °C (optimum, 40 °C), in 20-35 % (25 %) NaCl and pH 8-10 (pH 9). Mg not required for growth. The cells were lysed in distilled water and the minimum NaCl concentration that prevented cell lysis was 5 % w/v. The 16S rRNA gene sequence similarities between strain ESP3B_9 and JCM 10989 and ATCC 43099 were 96.53 and 96.25 % respectively. The similarities of the RNA polymerase subunit B gene between strain ESP3B_9 and JCM 10989 and ATCC 43099 were 84.47 and 84.9 % respectively. Genome sequencing revealed a genome size of 4.20 Mbp with DNA G+C content of 62.5 mol%. The major polar lipids were phosphotidylglycerol and phosphatidylglycerol phosphate methyl esters with minor amounts of unidentified lipids. The results of polyphasic analysis determined that strain ESP3B_9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is ESP3B_9 (MCC 3419=JCM 33002=KCTC 4279=CGMCC 1.16737).
从印度拉贾斯坦邦桑巴尔盐湖盐田分离到一株盐古菌（ESP3B_9）。细胞呈球形，不运动，革兰氏染色阴性，形成粉红色色素菌落。该菌株为需氧菌，能在 35-55°C（最佳温度为 40°C）、20-35%（25%）NaCl 和 pH8-10（pH9）条件下生长。生长不需要镁。细胞在蒸馏水中裂解，防止细胞裂解的最小 NaCl 浓度为 5%w/v。ESP3B_9 菌株与 JCM 10989 和 ATCC 43099 的 16S rRNA 基因序列相似性分别为 96.53%和 96.25%。ESP3B_9 菌株与 JCM 10989 和 ATCC 43099 的 RNA 聚合酶亚基 B 基因相似性分别为 84.47%和 84.9%。基因组测序显示其基因组大小为 4.20 Mbp，DNA G+C 含量为 62.5 mol%。主要极性脂为磷脂酰甘油和磷脂酰甘油磷酸酯甲基酯，还有少量未鉴定的脂类。多相分析结果表明，ESP3B_9 株代表了一个新的属种，建议命名为 sp. nov.。模式株为 ESP3B_9（MCC 3419=JCM 33002=KCTC 4279=CGMCC 1.16737）。
