National Centre for Cell Science, Pune, Maharashtra, 411007, India.
Abasaheb Garware College, Pune, Maharashtra, 411004, India.
Curr Microbiol. 2022 Jan 4;79(2):51. doi: 10.1007/s00284-021-02740-1.
A halophilic archaeon, designated strain LS1_42, was isolated from Sambhar Salt Lake, Rajasthan, India. Cells were non-motile, coccoid, Gram-stain-variable and present in irregular clusters with light pink pigmented colonies. The strain was strictly aerobic and able to grow without Mg. Growth of the strain LS1_42 was observed at 25-45 °C, pH 7.0-11.0 and NaCl concentrations of 10-35% (w/v). The nearest phylogenetic neighbor of strain LS1_42 was Natronococcus amylolyticus Ah-36 based on 16S rRNA and rpoB' genes with similarity of 95.4% and 91.9%, respectively. Phylogenetic analysis based on 16S rRNA gene, rpoB' gene and whole-genome sequences indicate that the strain LS1_42 belongs to the genus Natronococcus and is closely related to N. amylolyticus. The genome size was 5.38 Mb with 98.9% completeness. The DNA G + C content of the strain LS1_42 was 63.0 mol%. The average nucleotide identity, average amino acid identity and DNA-DNA hybridization values between LS1_42 and N. amylolyticus Ah-36 were 81.3%, 77.7% and 24.8%, respectively. The major polar lipids detected were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. On the basis of phenotypic, chemotaxonomic and genome-based analysis, strain LS1_42 represents a novel species within the genus Natronococcus, for which the name Natronococcus pandeyae sp. nov. is proposed. The type strain is LS1_42 (MCC 3654 = JCM 33003 = KCTC 4280 = CGMCC 1.16738).
一种嗜盐古菌，命名为 LS1_42 菌株，从印度拉贾斯坦邦的桑巴尔盐湖中分离得到。细胞是非运动性的、球形的、革兰氏染色可变的，存在于不规则的簇中，带有浅粉色的色素菌落。该菌株是严格需氧的，能够在没有镁的情况下生长。LS1_42 菌株的生长观察到在 25-45°C、pH7.0-11.0 和 NaCl 浓度为 10-35%（w/v）的条件下。根据 16S rRNA 和 rpoB'基因，LS1_42 菌株与 Natronococcus amylolyticus Ah-36 的最接近的系统发育近邻分别为 95.4%和 91.9%的相似性。基于 16S rRNA 基因、rpoB'基因和全基因组序列的系统发育分析表明，LS1_42 菌株属于 Natronococcus 属，与 Natronococcus amylolyticus 密切相关。基因组大小为 5.38 Mb，完整性为 98.9%。LS1_42 菌株的 DNA G+C 含量为 63.0 mol%。LS1_42 和 N. amylolyticus Ah-36 之间的平均核苷酸同一性、平均氨基酸同一性和 DNA-DNA 杂交值分别为 81.3%、77.7%和 24.8%。检测到的主要极性脂为磷脂酰甘油和磷脂酰甘油磷酸甲酯。基于表型、化学分类和基于基因组的分析，LS1_42 菌株代表了 Natronococcus 属中的一个新种，提议命名为 Natronococcus pandeyae sp. nov.。模式菌株为 LS1_42（MCC 3654=JCM 33003=KCTC 4280=CGMCC 1.16738）。
