Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran.
Extremophile Laboratory, Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1932-1936. doi: 10.1099/ijs.0.036921-0. Epub 2011 Oct 14.
A novel pale pink-pigmented halophilic archaeon, strain DC30(T), was isolated from Aran-Bidgol salt lake, a hypersaline playa in Iran. Cells of strain DC30(T) were non-motile and pleomorphic, from rods to triangular or disc-shaped. Strain DC30(T) required at least 1.7 M NaCl and 0.05 M MgCl(2) for growth (optimum, 3 M NaCl and 0.1 M MgCl(2)). The optimum pH and temperature for growth of strain DC30(T) were pH 7.5 and 40 °C, respectively, although it was capable of growth over pH and temperature ranges of 6.5-8.5 and 25-50 °C, respectively. Analysis of the 16S rRNA gene sequence showed that strain DC30(T) was a member of the family Halobacteriaceae. However, it had low 16S rRNA gene sequence similarities of 92.4%, 89.4% and 89.1% to the most closely related haloarchaeal taxa, the type species of the genera Halorubrum, Halogranum and Haloplanus, respectively. The DNA G+C content was 66.0 mol%. Phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester, common phospholipids found in haloarchaea, were present. Three minor phospholipids and one unidentified glycolipid were also observed. The only quinone present was MK-8(II-H(2)). The physiological, biochemical and phylogenetic differences between strain DC30(T) and other previously described genera of extremely halophilic archaea suggest that strain DC30(T) represents a novel species in a new genus within the family Halobacteriaceae, for which the name Halopenitus persicus gen. nov., sp. nov. is proposed. The type strain of Halopenitus persicus is DC30(T) ( = IBRC 10041(T) = KCTC 4046(T)).
一株新型淡粉色着色嗜盐古菌，菌株 DC30(T)，从伊朗高盐度干盐湖 Aran-Bidgol 盐湖中分离得到。菌株 DC30(T)细胞是非运动的，多形的，从杆状到三角形或盘状。菌株 DC30(T)的生长至少需要 1.7 M NaCl 和 0.05 M MgCl(2)(最佳生长条件为 3 M NaCl 和 0.1 M MgCl(2))。菌株 DC30(T)生长的最适 pH 值和温度分别为 pH 7.5 和 40°C，尽管它能够在 pH 值和温度范围分别为 6.5-8.5 和 25-50°C 之间生长。16S rRNA 基因序列分析表明，菌株 DC30(T)是盐杆菌科的一个成员。然而，它与最密切相关的嗜盐古菌分类群的 16S rRNA 基因序列相似性较低，分别为 92.4%、89.4%和 89.1%，分别为属 Halorubrum、Halogranum 和 Haloplanus 的模式种。DNA G+C 含量为 66.0 mol%。存在常见于嗜盐古菌的磷脂酰甘油和磷脂酰甘油磷酸甲酯。还观察到三种少量磷脂和一种未鉴定的糖脂。存在的唯一醌是 MK-8(II-H(2))。菌株 DC30(T)与其他先前描述的极端嗜盐古菌属之间的生理、生化和系统发育差异表明，菌株 DC30(T)代表盐杆菌科中一个新属的新型物种，建议将其命名为 Halopenitus persicus gen. nov.，sp. nov.。 Halopenitus persicus 的模式菌株为 DC30(T)( = IBRC 10041(T) = KCTC 4046(T))。
