Xie Xiang-Li, Wei Yi, Song Yan-Yue, Pan Guan-Ming, Chen Li-Na, Wang Gang, Zhang Shi-Hong
College of Plant Sciences, Jilin University, Changchun, China.
School of Life Sciences, Henan University, Kaifeng, China.
Front Microbiol. 2020 Jan 6;10:2994. doi: 10.3389/fmicb.2019.02994. eCollection 2019.
Fungal sexual development requires the involvement of a large number of functional genes. Fungal genes encoding sexual differentiation process proteins (), , have been known for decades. / and its homologs function as oligopeptide transporters (OPTs), yet their roles in reproduction are unknown. Here, we genetically analyzed all four homologs in the sexual species and asexual species Using single gene deletion mutants, we found that () participated in asexual sporulation, whereas the other homologs participated in sexual morphogenesis. In complementary tests, - restored sporulation defects in deletion strains (Δ), and their translated proteins, which were localized onto the cytomembrane, possessed OPT activity. Interestingly, accumulated at the top of the hyphae played a distinct role in determining the sexual cycle, glutathione transport, and lifespan shortening. A unique 72nt-insertion fragment (72INS) was discovered in . Biological analysis of the 72INS deletion and DsRED-tagged fusion strains implied the involvement of 72INS in fungal growth and development. In contrast to , which only contributes to conidial production, the three s play important roles in sexual and asexual reproduction, and harbors a unique functional 72INS that initiates sexual morphogenesis.
真菌有性发育需要大量功能基因的参与。编码有性分化过程蛋白（）、、的真菌基因已为人所知数十年。/及其同源物作为寡肽转运蛋白（OPTs）发挥作用，但其在繁殖中的作用尚不清楚。在这里，我们对有性物种和无性物种中的所有四个同源物进行了遗传分析。使用单基因缺失突变体，我们发现（）参与无性孢子形成，而其他同源物参与有性形态发生。在互补试验中，-恢复了缺失菌株（Δ）中的孢子形成缺陷，并且它们的翻译蛋白定位于细胞膜上，具有OPT活性。有趣的是，积累在菌丝顶端的在决定性周期、谷胱甘肽转运和寿命缩短方面发挥了独特作用。在中发现了一个独特的72nt插入片段（72INS）。对72INS缺失和DsRED标记融合菌株的生物学分析表明72INS参与真菌生长和发育。与仅有助于分生孢子产生的不同，三个在有性和无性繁殖中发挥重要作用，并且含有启动有性形态发生的独特功能性72INS。
