Carmo-Fonseca M
Department of Cell Biology, Gulbenkian Institute of Science, Oeiras, Portugal.
Cell Biol Int Rep. 1988 Aug;12(8):607-20. doi: 10.1016/0309-1651(88)90153-1.
The major rat ventral prostate androgen-dependent nuclear proteins were studied using isolated nuclei, nuclear matrix and nuclear envelope fractions. Nuclear and subnuclear fractions obtained were characterized by electron microscopy and SDS-polyacrylamide gel electrophoresis. A group of approximately 20 kDa peptides is demonstrated to be present in nuclei, nuclear matrices and nuclear envelopes from normal prostate. Time course experiments indicate that the 20 kDa peptides become drastically reduced after 7 or 10 days following castration and are incompletely restored after 3 daily testosterone injections. Lectin binding studies demonstrate that the 20 kDa peptides bind both to Concanavalin A and Wheat Germ Agglutinin. These peptides represent the major nuclear Concanavalin A binding glycoproteins from normal prostate nuclei and nuclear matrices.
利用分离的细胞核、核基质和核被膜组分，对大鼠腹侧前列腺主要的雄激素依赖性核蛋白进行了研究。通过电子显微镜和SDS-聚丙烯酰胺凝胶电泳对所获得的细胞核和亚核组分进行了表征。结果表明，一组分子量约为20 kDa的肽存在于正常前列腺的细胞核、核基质和核被膜中。时间进程实验表明，去势后7天或10天，这些20 kDa的肽显著减少，在每天注射睾酮3次后未完全恢复。凝集素结合研究表明，这些20 kDa的肽既与伴刀豆球蛋白A结合，也与麦胚凝集素结合。这些肽代表了来自正常前列腺细胞核和核基质的主要核伴刀豆球蛋白A结合糖蛋白。
