Suematsu Naoya, Mizuno Takeo
Zoological Institute, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan.
Department of Biology, Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-01, Japan.
Dev Growth Differ. 1991 Aug;33(4):379-389. doi: 10.1111/j.1440-169X.1991.00379.x.
When urinary bladder epithelia of rats were grown in association with fetal urogenital sinus mesenchyme, prostatic morphogenesis was induced. The epithelial proteins were examined by HPLC fractionation followed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). More than 500 bands of silver-stained epithelial proteins were analyzed. The glandular epithelia induced from both adult and fetal bladder epithelia lost all of the 7 bladder-specific bands (BE 1-7) in most recombinants and expressed a number of prostate-specific bands. Among the 18 bands commonly found in all prostatic lobes, 13 (PE 4, 7-18) were constantly and 3 (PE 1-3) were sporadically detected, while the other 2 (PE 5 and 6) bands were not detected when the adult epithelium was used in recombination. Among the 7 prostatic lobe-specific bands (vPE 14, dPE 1-3), most of them were detected when the fetal epithelium was used, while few of them when the adult epithelium was used. These results demonstrate that prostatic morphogenesis induced in the bladder epithelium was associated with most of biochemical features of prostate. In addition to the biochemical study, histological examination revealed that the prostatic differentiation was more complete in the fetal bladder epithelium than the adult one.
当将大鼠膀胱上皮与胎儿泌尿生殖窦间充质联合培养时，可诱导前列腺形态发生。通过高效液相色谱分离，随后进行十二烷基硫酸钠（SDS）-聚丙烯酰胺凝胶电泳（PAGE）来检测上皮蛋白。分析了500多条银染上皮蛋白条带。在大多数重组体中，由成年和胎儿膀胱上皮诱导产生的腺上皮失去了所有7条膀胱特异性条带（BE 1-7），并表达了一些前列腺特异性条带。在所有前列腺叶中常见的18条带中，13条（PE 4, 7-18）持续被检测到，3条（PE 1-3）偶尔被检测到，而当成年上皮用于重组时，另外2条带（PE 5和6）未被检测到。在7条前列腺叶特异性条带（vPE 14, dPE 1-3）中，使用胎儿上皮时大多数能被检测到，而使用成年上皮时则很少能检测到。这些结果表明，膀胱上皮中诱导的前列腺形态发生与前列腺的大多数生化特征相关。除了生化研究外，组织学检查还显示，胎儿膀胱上皮中的前列腺分化比成年膀胱上皮更完全。
