Analysis of Epithelial Protein Profiles of Prostatic Glands Induced Heterotypically in the Bladder Epithelium of the Rat: (mesenchymal-epithelial interaction/prostate-specific/heterotypic differentiation/urinary bladder/urogenital sinus).

When urinary bladder epithelia of rats were grown in association with fetal urogenital sinus mesenchyme, prostatic morphogenesis was induced. The epithelial proteins were examined by HPLC fractionation followed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). More than 500 bands of silver-stained epithelial proteins were analyzed. The glandular epithelia induced from both adult and fetal bladder epithelia lost all of the 7 bladder-specific bands (BE 1-7) in most recombinants and expressed a number of prostate-specific bands. Among the 18 bands commonly found in all prostatic lobes, 13 (PE 4, 7-18) were constantly and 3 (PE 1-3) were sporadically detected, while the other 2 (PE 5 and 6) bands were not detected when the adult epithelium was used in recombination. Among the 7 prostatic lobe-specific bands (vPE 14, dPE 1-3), most of them were detected when the fetal epithelium was used, while few of them when the adult epithelium was used. These results demonstrate that prostatic morphogenesis induced in the bladder epithelium was associated with most of biochemical features of prostate. In addition to the biochemical study, histological examination revealed that the prostatic differentiation was more complete in the fetal bladder epithelium than the adult one.