van Eijk H M, van der Heijden M A, van Berlo C L, Soeters P B
University Hospital Maastricht, Department of Surgery, The Netherlands.
Clin Chem. 1988 Dec;34(12):2510-3.
This inexpensive method for fully automated amino acid analysis combines the advantages of automated precolumn derivatization with o-phthaldialdehyde and favorable analytical conditions to separate and quantify 30 amino acids found in normal plasma. The system can run unattended for almost four days, during which the data are processed automatically by a personal computer and a maximum of 76 samples and 19 standards can be processed (cycle time per analysis: 55 min). Only 1 microL of deproteinized plasma is required per analysis. Coefficients of variation for retention times and areas measured for all relevant amino acids are less than 1% and 3%, respectively. The system described is well suited for quick, sensitive operation in daily practice.
这种用于全自动氨基酸分析的低成本方法结合了邻苯二甲醛自动柱前衍生化的优点以及有利的分析条件，可分离并定量正常血浆中发现的30种氨基酸。该系统可以无人值守运行近四天，在此期间数据由个人计算机自动处理，最多可处理76个样本和19个标准品（每次分析的循环时间：55分钟）。每次分析仅需1微升脱蛋白血浆。所有相关氨基酸的保留时间和峰面积的变异系数分别小于1%和3%。所描述的系统非常适合日常实践中的快速、灵敏操作。
