The Second Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China.
Department of Geriatrics, Zhejiang Provincial Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou, Zhejiang 310013, P.R. China.
Mol Med Rep. 2020 Feb;21(2):641-648. doi: 10.3892/mmr.2019.10891. Epub 2019 Dec 17.
Crocetin, a natural compound, has been demonstrated to exhibit beneficial effects in cardiovascular diseases. Previous studies demonstrated that crocetin reduced ischemia/reperfusion (I/R) injury by attenuating cytotoxicity and cellular apoptosis. However, the previous mechanistic studies did not fully elucidate its pharmacological effects on cardiac damage, especially I/R injury. The present study verified its cardioprotective effects in a Langendorff perfusion system, an ex vivo model of I/R. It was demonstrated that crocetin significantly attenuated the activities of pro‑inflammatory cytokines and nuclear factor erythroid‑2 related factor 2 (Nrf2)/heme oxygenase‑1 signaling. The present study provided novel insight that crocetin regulated the unfolded protein response (UPR) and decreased associated protein levels to protect the heart. Furthermore, it was identified that Nrf2 played a key role in the cardioprotective effect of crocetin by attenuating inflammation and the UPR.
西红花酸是一种天然化合物，已被证明对心血管疾病有有益的作用。先前的研究表明，西红花酸通过减轻细胞毒性和细胞凋亡来减轻缺血/再灌注（I/R）损伤。然而，以前的机制研究并没有完全阐明其对心脏损伤，特别是 I/R 损伤的药理学作用。本研究在 Langendorff 灌注系统（I/R 的离体模型）中验证了西红花酸的心脏保护作用。结果表明，西红花酸显著减弱了促炎细胞因子的活性和核因子红细胞 2 相关因子 2（Nrf2）/血红素加氧酶 1 信号通路。本研究提供了新的见解，即西红花酸通过调节未折叠蛋白反应（UPR）和降低相关蛋白水平来保护心脏。此外，研究还发现 Nrf2 通过减轻炎症和 UPR 发挥关键作用，从而增强了西红花酸的心脏保护作用。
