Hanneman E, Trevarrow B, Metcalfe W K, Kimmel C B, Westerfield M
Institute of Neuroscience, University of Oregon, Eugene 97403.
Development. 1988 May;103(1):49-58. doi: 10.1242/dev.103.1.49.
In the ventral hindbrain and spinal cord of zebrafish embryos, the first neurones that can be identified appear as single cells or small clusters of cells, distributed periodically at intervals equal to the length of a somite. In the hindbrain, a series of neuromeres of corresponding length is present, and the earliest neurones are located in the centres of each neuromere. Young neurones within both the hindbrain and spinal cord were identified in live embryos using Nomarski optics, and histochemically by labelling for acetylcholinesterase activity and expression of an antigen recognized by the monoclonal antibody zn-1. Among them are individually identified hindbrain reticulospinal neurones and spinal motoneurones. These observations suggest that early development in these regions of the CNS reflects a common segmental pattern. Subsequently, as more neurones differentiate, the initially similar patterning of the cells in these two regions diverges. A continuous longitudinal column of developing neurones appears in the spinal cord, whereas an alternating series of large and small clusters of neurones is present in the hindbrain.
在斑马鱼胚胎的腹侧后脑和脊髓中，最早可识别的神经元表现为单个细胞或小细胞簇，以等于一个体节长度的间隔周期性分布。在后脑中，存在一系列长度相应的神经节段，最早的神经元位于每个神经节段的中心。利用诺马斯基光学显微镜在活胚胎中识别后脑和脊髓内的年轻神经元，并通过乙酰胆碱酯酶活性标记和单克隆抗体zn-1识别的抗原表达进行组织化学鉴定。其中包括单独鉴定的后脑网状脊髓神经元和脊髓运动神经元。这些观察结果表明，中枢神经系统这些区域的早期发育反映了一种共同的节段模式。随后，随着更多神经元分化，这两个区域中最初相似的细胞模式出现分歧。脊髓中出现了一列连续的发育中的神经元纵列，而后脑中则存在大小交替的神经元簇系列。
