大鼠晶状体中的葡萄糖通量与吡啶二核苷酸的氧化还原状态

Glucose flux and the redox state of pyridine dinucleotides in the rat lens.

作者信息

Cheng H M, González R G, von Saltza I, Chylack L T, Hutson N J

机构信息

Howe Laboratory of Ophthalmology, Harvard Medical School, Boston, MA 02114.

出版信息

Exp Eye Res. 1988 Jun;46(6):947-52. doi: 10.1016/s0014-4835(88)80046-0.

DOI:10.1016/s0014-4835(88)80046-0

PMID:3197763

Abstract

The concentration of alpha-glycerophosphate (GP) appeared to increase and readily reached a new steady state in lenses incubated with KCN or under hyperglucosic condition. This increase can be explained by the change in NADH/NAD ratio under each condition. The relative ratio of pyridine dinucleotides (NADH/NAD) was calculated from equilibrium equations of two NAD-linked enzymes, lactate dehydrogenase (LDH) and alpha-glycerophosphate dehydrogenase (GPDH). The change in NADH/NAD ratio based on biochemical assays correlates well with that estimated from GPDH reaction. This indicates that measurements of pyridine dinucleotides (e.g. in vivo redox fluorometry) can be used to demonstrate lens metabolic status.

摘要

在与氰化钾一起孵育的晶状体中或在高糖条件下，α-甘油磷酸（GP）的浓度似乎会升高，并迅速达到新的稳态。这种升高可以通过每种条件下NADH/NAD比值的变化来解释。吡啶二核苷酸（NADH/NAD）的相对比值是根据两种NAD连接酶，即乳酸脱氢酶（LDH）和α-甘油磷酸脱氢酶（GPDH）的平衡方程计算得出的。基于生化分析的NADH/NAD比值变化与从GPDH反应估计的变化密切相关。这表明吡啶二核苷酸的测量（例如体内氧化还原荧光测定法）可用于证明晶状体的代谢状态。