常用于细胞培养的各种血清中的维生素及其他代谢物。
Vitamins and other metabolites in various sera commonly used for cell culturing.
作者信息
Baker H, DeAngelis B, Frank O
机构信息
New Jersey Medical School, Department of Preventive Medicine and Community Health, Newark 07107.
出版信息
Experientia. 1988 Dec 1;44(11-12):1007-10. doi: 10.1007/BF01939904.
Many cell culture media use different sera to enhance growth. We assayed vitamins and some related metabolites in different sera and identified the concentration of: thiamin, biotin, folates, riboflavin, pantothenates, nicotinates, vitamins B6, B12, A, E, C, and carotenes and some related metabolites: biopterins, free inositol, free and total choline, total carnitines in chicken, horse, rabbit, goat, pig, calf, newborn calf, fetal calf and human sera. Results indicate that vitamin and metabolite content of different sera vary. Such variations could produce fluctuant effects on cell culturings if the metabolite content of the serum is not documented.
许多细胞培养基使用不同的血清来促进生长。我们检测了不同血清中的维生素和一些相关代谢物，并确定了以下物质的浓度：硫胺素、生物素、叶酸、核黄素、泛酸盐、烟酸盐、维生素B6、B12、A、E、C、胡萝卜素以及一些相关代谢物：生物蝶呤、游离肌醇、游离胆碱和总胆碱、鸡肉、马、兔、山羊、猪、小牛、新生小牛、胎牛和人血清中的总肉碱。结果表明，不同血清中的维生素和代谢物含量各不相同。如果血清中的代谢物含量没有记录，这些差异可能会对细胞培养产生波动影响。
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