Kluknavsky Michal, Balis Peter, Skratek Martin, Manka Jan, Bernatova Iveta
Slovak Academy of Sciences, Centre of Experimental Medicine, Institute of Normal and Pathological Physiology, 813 71 Bratislava, Slovakia.
Slovak Academy of Sciences, Institute of Measurement Science, 841 04 Bratislava, Slovakia.
Antioxidants (Basel). 2020 Jan 23;9(2):96. doi: 10.3390/antiox9020096.
This study investigated the effects of (-)-epicatechin (Epi) in young male borderline hypertensive rats (BHR) during two weeks of treatment (Epi group, 100 mg/kg/day p.o.) and two weeks post treatment (PE group). Epi reduced blood pressure (BP), which persisted for two weeks post treatment. This was associated with delayed reduction of anxiety-like behaviour. Epi significantly increased nitric oxide synthase (NOS) activities in the aorta and left heart ventricle (LHV) vs. the age-matched controls without affecting the brainstem and frontal neocortex. Furthermore, Epi significantly reduced the superoxide production in the aorta and relative content of iron-containing compounds in blood. Two weeks post treatment, the NOS activities and superoxide productions in the heart and aorta did not differ from the age-matched controls. The gene expressions of the NOSs (S, ), nuclear factor erythroid 2-related factor 2 (), and peroxisome proliferator-activated receptor-γ (-γ) remained unaltered in the aorta and LHV of the Epi and PE groups. In conclusion, while Epi-induced a decrease of the rats' BP persisted for two weeks post treatment, continuous Epi treatments seem to be necessary for maintaining elevated NO production as well as redox balance in the heart and aorta without changes in the , , and -γ gene expressions.
本研究调查了（-）-表儿茶素（Epi）对年轻雄性边缘性高血压大鼠（BHR）在为期两周的治疗期间（Epi组，口服100mg/kg/天）以及治疗后两周（PE组）的影响。Epi降低了血压（BP），且在治疗后两周仍持续存在。这与焦虑样行为的延迟减轻有关。与年龄匹配的对照组相比，Epi显著增加了主动脉和左心室（LHV）中的一氧化氮合酶（NOS）活性，而不影响脑干和额叶新皮质。此外，Epi显著降低了主动脉中的超氧化物生成以及血液中含铁化合物的相对含量。治疗后两周，心脏和主动脉中的NOS活性和超氧化物生成与年龄匹配的对照组无差异。Epi组和PE组的主动脉和LHV中，NOS（S、 ）、核因子红细胞2相关因子2（ ）和过氧化物酶体增殖物激活受体-γ（-γ）的基因表达保持不变。总之，虽然Epi诱导的大鼠血压降低在治疗后两周持续存在，但持续的Epi治疗似乎对于维持心脏和主动脉中升高的NO生成以及氧化还原平衡是必要的，而 、 和 -γ基因表达没有变化。
