Cummins Max Laurence, Hamidian Mohammad, Djordjevic Steven Philip
The Ithree Institute, University of Technology Sydney, Ultimo, NSW 2007, Australia.
Australian Centre for Genomic Epidemiological Microbiology, University of Technology Sydney, PO Box 123, Broadway, NSW 2007, Australia.
Microorganisms. 2020 Jan 23;8(2):161. doi: 10.3390/microorganisms8020161.
genomic island 1 (SGI1) is an integrative mobilisable element that plays an important role in the capture and spread of multiple drug resistance. To date, SGI1 has been found in clinical isolates of serovars, , , , , and recently in . SGI1 preferentially targets the 3´-end of , a conserved gene found in the Enterobacteriaceae and among members of the Gammaproteobacteria. It is, therefore, hypothesised that SGI1 and SGI1-related elements (SGI1-REs) may have been acquired by diverse bacterial genera. Here, Bitsliced Genomic Signature Indexes (BIGSI) was used to screen the NCBI Sequence Read Archive (SRA) for putative SGI1-REs in Gammaproteobacteria. Novel SGI-REs were identified in diverse genera including spp, spp, and spp and in two additional isolates of . An extensively drug-resistant human clonal lineage of carrying an SGI1-RE in the United Kingdom and an SGI1-RE that lacks a class 1 integron were also identified. These findings provide insight into the origins of this diverse family of clinically important genomic islands and expand the knowledge of the potential host range of SGI1-REs within the Gammaproteobacteria.
基因组岛1（SGI1）是一种可整合移动元件，在多重耐药性的获取和传播中发挥重要作用。迄今为止，已在血清型 、 、 、 、 、 的临床分离株中发现SGI1，最近在 中也有发现。SGI1优先靶向 基因的3´端， 是肠杆菌科和γ-变形菌纲成员中发现的一个保守基因。因此，推测SGI1和SGI1相关元件（SGI1-REs）可能已被多种细菌属获得。在此，使用位片基因组特征索引（BIGSI）在NCBI序列读取存档（SRA）中筛选γ-变形菌纲中假定的SGI1-REs。在包括 属、 属和 属等不同属以及另外两株 的分离株中鉴定出新型SGI-REs。还鉴定出一株在英国携带SGI1-RE的广泛耐药人类克隆谱系的 菌以及一个缺乏1类整合子的SGI1-RE。这些发现为这一临床上重要的基因组岛多样化家族的起源提供了见解，并扩展了γ-变形菌纲内SGI1-REs潜在宿主范围的知识。
