Wagner G F, Fenwick J C, Park C M, Milliken C, Copp D H, Friesen H G
Department of Physiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada.
Gen Comp Endocrinol. 1988 Nov;72(2):237-46. doi: 10.1016/0016-6480(88)90206-7.
This is a comparative study of the glycoprotein hormone, teleocalcin, from the corpuscles of Stannius of sockeye (Oncorhynchus nerka) and coho (O. kisutch) salmon. Coho teleocalcin was purified by the same procedures used previously to obtain sockeye teleocalcin and was obtained in a comparable yield. Both salmon teleocalcins had the same molecular weight as estimated by sodium dodecyl sulfate-electrophoresis and both appeared to have the structure of disulfide-linked oligomers. The two hormones were similar on the basis of amino acid and carbohydrate composition and shared 95% homology in the first 40 residues on the N-terminal. The salmon teleocalcins also shared 80% homology with the predicted 1-40 N-terminal sequence from Australian eels (Anguilla australis). Both teleocalcins had potent inhibitory effects on gill calcium uptake in intact rainbow trout (Salmo gairdneri). However, these effects were observed only at the peak in the calcium uptake cycle that is displayed by this species. In North American eels (A. rostrata), the acute administration of both teleocalcins caused significant inhibition of gill calcium uptake without any concomitant changes in plasma calcium levels or other plasma electrolytes. In 4- and 7-day stanniectomy (STX) eels, the acute administration of coho teleocalcin significantly reduced or completely abolished the accelerated gill calcium transport that occurs postoperatively, with no concomitant changes in plasma electrolytes or post-STX hypercalcemia. These experiments provide further evidence that teleocalcin is a regulator of gill calcium transport and has no acute hypocalcemic effects in fish.
这是一项对红大麻哈鱼（Oncorhynchus nerka）和银大麻哈鱼（O. kisutch）斯坦尼小体中的糖蛋白激素远钙素进行的比较研究。银大麻哈鱼远钙素通过先前用于获取红大麻哈鱼远钙素的相同程序进行纯化，且获得了相当的产量。通过十二烷基硫酸钠电泳估计，两种大麻哈鱼远钙素具有相同的分子量，并且两者似乎都具有二硫键连接的寡聚体结构。基于氨基酸和碳水化合物组成，这两种激素相似，并且在N端的前40个残基中具有95%的同源性。大麻哈鱼远钙素与澳大利亚鳗鱼（Anguilla australis）预测的1-40 N端序列也有80%的同源性。两种远钙素对完整虹鳟鱼（Salmo gairdneri）鳃对钙的摄取均有强烈的抑制作用。然而，这些作用仅在该物种显示的钙摄取周期的峰值时观察到。在北美鳗鱼（A. rostrata）中，两种远钙素的急性给药均导致鳃对钙的摄取受到显著抑制，而血浆钙水平或其他血浆电解质没有任何伴随变化。在4天和7天的切除斯坦尼小体（STX）的鳗鱼中，银大麻哈鱼远钙素的急性给药显著降低或完全消除了术后出现的加速鳃钙转运，而血浆电解质或STX后高钙血症没有伴随变化。这些实验进一步证明远钙素是鳃钙转运的调节剂，并且在鱼类中没有急性降钙作用。
