Citarella F, Tripodi M, Fantoni A, Bernardi F, Romeo G, Rocchi M
Dipartimento di Biopatologia Umana, Università di Roma, La Sapienza, Italy.
Hum Genet. 1988 Dec;80(4):397-8. doi: 10.1007/BF00273661.
Human coagulation factor XII (fXII), a serine protease synthesized in liver and active in plasma, is involved in a wide variety of functions, including blood coagulation, fibrinolysis, bradykinin and complement activation. A complementary DNA (597 bp) encoding amino acid -16 to amino acid 183 of fXII protein was used to determine the chromosomal location of the fXII gene. DNAs from hamster-human somatic cell hybrids were digested with restriction enzymes and hybridized with the fXII cDNA. By the Southern method it was shown that restriction fragments able to hybridize to fXII cDNA are present only in DNA extracted from clones retaining human chromosome 5.
人凝血因子XII（fXII）是一种在肝脏中合成并在血浆中具有活性的丝氨酸蛋白酶，参与多种功能，包括血液凝固、纤维蛋白溶解、缓激肽和补体激活。一个编码fXII蛋白氨基酸-16至氨基酸183的互补DNA（597 bp）被用于确定fXII基因的染色体定位。用限制性内切酶消化仓鼠-人类体细胞杂种的DNA，并与fXII cDNA杂交。通过Southern方法表明，能够与fXII cDNA杂交的限制性片段仅存在于从保留人类5号染色体的克隆中提取的DNA中。
