Connecticut Convergence Institute for Translation in Regenerative Engineering, University of Connecticut Health Center, Farmington, Connecticut.
Raymond and Beverly Sackler Center for Biomedical, Biological, Physical and Engineering Sciences, University of Connecticut Health Center, Farmington, Connecticut.
J Biomed Mater Res A. 2020 May;108(5):1136-1143. doi: 10.1002/jbm.a.36888. Epub 2020 Feb 3.
Acute traumatic nail injury treatment repair procedures are commonly conducted in emergency departments and primary care offices. Current repair methods use nail splints that are inserted within the nail root to prevent the fusion of the proximal nail fold and the matrix tissue. Splints provide a protective barrier overlying the nail bed soft tissue during recovery periods, but uncertain prognoses (i.e., aesthetic and functional disadvantages) reveal a need for improved nail repair techniques. Nail splints are not specifically designed for nail organ restoration via biological mechanisms, thus, a clinical application that utilizes regenerative engineering techniques can prove useful in improving the nail injury prognoses. Using the coaxial electrospinning method, hybrid poly(lactide-co-glycolide) (PLGA) (85:15) and gelatin fibrous scaffolds (Hybrid PLGA shell, gelatin core and Hybrid : gelatin shell, PLGA core) with average fiber diameters of 540 ± 118 and 2,215 ± 1,135 nm, respectively, were produced and successful encapsulation of core fibers was observed. Furthermore, nail stem cells expressing stem cell characteristic markers CD90, CD29, and Lgr6 showed preferred attachment to Hybrid scaffolds after 24 hr. Overall, an in vitro regenerative engineered nail matrix may aid to improve the cosmetic appearance and function of injured nail organs post-traumatic injury.
急性外伤性指甲损伤的治疗修复程序通常在急诊科和初级保健办公室进行。目前的修复方法使用指甲夹板插入指甲根部，以防止近端甲褶和基质组织融合。夹板在恢复期为指甲床软组织提供了一个保护屏障，但不确定的预后（即美学和功能方面的劣势）表明需要改进指甲修复技术。指甲夹板不是专门为通过生物机制修复指甲器官而设计的，因此，利用再生工程技术的临床应用可能有助于改善指甲损伤的预后。使用同轴电纺方法，分别制备出平均纤维直径为 540±118nm 和 2215±1135nm 的混合聚（乳酸-共-乙醇酸）（85:15）和明胶纤维支架（Hybrid PLGA 壳，明胶核和 Hybrid：明胶壳，PLGA 核），并观察到芯纤维的成功包封。此外，表达干细胞特征标志物 CD90、CD29 和 Lgr6 的指甲干/祖细胞在孵育 24 小时后优先附着于 Hybrid 支架上。总的来说，体外再生工程指甲基质可能有助于改善外伤性指甲损伤后的指甲器官的美容外观和功能。
