Department of Plant Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.
Joint International Research Laboratory of Metabolic and Developmental Sciences, Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Microsc Res Tech. 2020 May;83(5):541-550. doi: 10.1002/jemt.23443. Epub 2020 Jan 25.
Taxonomy of the genus Berberis is quite complex, due to overlapping morphological characters, making it very difficult to differentiate the species within the genus. In order to resolve this taxonomic complexity, the foliar anatomy of 10 Berberis L. species was carried out, for the first time from Pakistan, using light microscopy (LM). Significant variation in terms of epidermal cells shape, size, cell wall pattern, and stomata type was observed. B. baluchistanica has the largest epidermal cells, Adaxial: length = 45-(53.9 ± 3.6)-62.5 μm; and width = 22.5-(26.3 ± 1.3)-30 μm; Abaxial: length = 37.5-(43.25 ± 2.5)-50 μm; and width = 20-(22.6 ± 0.8)-25. The highest number of stomata was observed in B. glaucocarpa as 62 on the abaxial surface while the lowest number of stomata was recorded in B. baluchistanica as 8 on the adaxial surface. Of 10 investigated species, 6 possess anomocytic type stomata, while 2 species that is, B. aitchisonii and B. parkeriana have both anomocytic and anisocytic stomata while B. baluchistanica and B. calliobotrys have only paracytic type stomata. The highest number of cells per unit area was present on the adaxial surface of B. calliobotrys ranging from 245-(252.4)-260 followed by B. parkeriana with 209-(227.8)-250 on the abaxial surface. Stomatal index (SI) also varied considerably and was the lowest (2.6) percentage in B. baluchistanica and highest (31.9) percentage in B. kunawurensis. A taxonomic key based on micro-morphological characters is provided for species identification.
由于形态特征重叠，贯叶连翘属的分类非常复杂，使得属内物种的区分变得非常困难。为了解决这个分类复杂性问题，首次从巴基斯坦利用光学显微镜（LM）对 10 种贯叶连翘属植物的叶解剖结构进行了研究。观察到表皮细胞形状、大小、细胞壁模式和气孔类型方面存在显著变化。B. baluchistanica 的表皮细胞最大，上表皮：长度=45-（53.9±3.6）-62.5μm；宽度=22.5-（26.3±1.3）-30μm；下表皮：长度=37.5-（43.25±2.5）-50μm；宽度=20-（22.6±0.8）-25μm。在背面观察到的气孔数最多，为 62 个，而在正面观察到的气孔数最少，为 B. baluchistanica 的 8 个。在 10 种被研究的物种中，有 6 种具有不等细胞型气孔，而 2 种，即 B. aitchisonii 和 B. parkeriana 具有不等细胞型和平列细胞型气孔，而 B. baluchistanica 和 B. calliobotrys 只有副卫细胞型气孔。B. calliobotrys 单位面积上的细胞数最多，上表皮为 245-（252.4）-260，其次是 B. parkeriana，下表皮为 209-（227.8）-250。气孔指数（SI）也有很大差异，B. baluchistanica 的 SI 最低（2.6），B. kunawurensis 的 SI 最高（31.9）。提供了基于微观形态特征的分类学关键特征，以用于物种鉴定。
