Laboratory of Pharmacology, Department of Drug Sciences, and LAQV@REQUIMTE, Faculty of Pharmacy, University of Porto, Portugal; Unité de Recherche: Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Département de Chimie, Université des Frères Mentouri Constantine 1, 25000, Constantine, Algeria.
Unité de Recherche: Valorisation des Ressources Naturelles, Molécules Bioactives et Analyses Physicochimiques et Biologiques, Département de Chimie, Université des Frères Mentouri Constantine 1, 25000, Constantine, Algeria; Laboratoire de Physiologie Animale, Département de Physiologie Animale, Université des Frères Mentouri Constantine 1, 25000, Constantine, Algeria.
J Ethnopharmacol. 2020 Apr 24;252:112613. doi: 10.1016/j.jep.2020.112613. Epub 2020 Jan 23.
Cymbopogon schoenanthus (C. schoenanthus) and Helianthemum lippii (H. lippii) are Saharan species found in the South West of Algeria, in the region of Bechar. Both plants are used in traditional medicine to treat gastrointestinal disorders.
The aim of our study was to characterize the composition of the ethyl acetate (EtOAc) and n-Butanol (n-BuOH) extracts of C. schoenanthus and H. lippii, and to elucidate and compare their effect on the reactivity of the rat distal colon.
The plants were macerated in a hydroalcoholic solution. After concentration, the aqueous solutions of the residues were submitted to liquid-liquid extractions to obtain EtOAc and n-BuOH extracts. The phenolic and flavonoid content of the extracts was determined by high performance liquid chromatography coupled with mass spectrometry with a time of flight analyzer (HPLC-TOF/MS). The effect of the extracts was tested on the rat distal colon, namely on the basal tone and on KCl- and Ach-induced precontracted preparations.
HPLC-TOF/MS identified 32 phenols and flavonoids in the extracts. The four extracts relaxed the rat distal colon, the effect being noticed on the basal tone and on the KCl- and Ach-induced precontractions. The EtOAc and the n-BuOH extracts of H. lippii decreased the basal tone of the rat distal colon more markedly than the correspondent extracts of C. schoenanthus. Moreover, the n-BuOH extract of C. schoenanthus decreased the basal tone more markedly than the EtOAc extract of this plant but there was no difference between extracts of H. lippii. The EtOAc extracts of both C. schoenanthus and H. lippii totally reverted both the KCl- and the Ach-induced precontraction of the rat distal colon. However, the n-BuOH extracts of the two plants reverted the Ach-precontracted colon but not the colon that has been precontracted with KCl.
Extracts of H. lippii contain a higher level of phenols compared to the extracts of C. schoenanthus. All extracts of C. schoenanthus and H. lippii caused marked relaxation of the isolated rat distal colon, either when applied directly or when tested over KCl- and Ach-induced precontraction. These results give support to the use of C. shoenanthus and H. lippii in traditional medicine, namely for gastrointestinal diseases.
香茅（Cymbopogon schoenanthus）和半日花（Helianthemum lippii）是在阿尔及利亚西南部、贝沙尔地区发现的撒哈拉物种。这两种植物都在传统医学中用于治疗胃肠道疾病。
我们的研究旨在描述香茅和半日花的乙酸乙酯（EtOAc）和正丁醇（n-BuOH）提取物的组成，并阐明和比较它们对大鼠远端结肠反应性的影响。
将植物在水醇溶液中浸渍。浓缩后，将残渣的水溶液进行液液萃取以获得 EtOAc 和 n-BuOH 提取物。通过高效液相色谱-飞行时间质谱联用仪（HPLC-TOF/MS）测定提取物的酚类和类黄酮含量。测试提取物对大鼠远端结肠的影响，即在基础张力和 KCl 和 Ach 诱导的预收缩制剂上的影响。
HPLC-TOF/MS 在提取物中鉴定出 32 种酚类和类黄酮。四种提取物均松弛大鼠远端结肠，对基础张力和 KCl 和 Ach 诱导的预收缩均有作用。半日花的 EtOAc 和 n-BuOH 提取物比香茅的相应提取物更显著地降低大鼠远端结肠的基础张力。此外，香茅的 n-BuOH 提取物比其 EtOAc 提取物更显著地降低基础张力，但半日花的提取物之间没有差异。两种植物的 EtOAc 提取物完全逆转了大鼠远端结肠的 KCl 和 Ach 诱导的预收缩。然而，两种植物的 n-BuOH 提取物仅逆转 Ach 预收缩的结肠，而不逆转 KCl 预收缩的结肠。
半日花的提取物比香茅的提取物含有更高水平的酚类化合物。香茅和半日花的所有提取物均引起分离的大鼠远端结肠明显松弛，无论是直接应用还是在 KCl 和 Ach 诱导的预收缩上测试时。这些结果支持香茅和半日花在传统医学中的应用，特别是用于胃肠道疾病。
