Bertella Anis, Smadi Abla, Benhabrou Hakim, Salvador Diana, Wrona Magdalena, Oliveira Helena, Sidaoui Abouamama, Gavril-Luminita Georgiana, Pinto Diana C G A, Olewnik-Kruszkowska Ewa, Nerín Cristina, Silva Artur M S, Bitam Fatma
Department of Molecular and Cellular Biology, Faculty of Life and Nature Sciences, Abbes Laghrour University Khenchela, BP 1252 Road of Batna, Khenchela 40004, Algeria.
Laboratory of Chemistry and Environmental Chemistry (LCCE), Department of Chemistry, Faculty of Matter Sciences, University of Batna 1, Batna 05000, Algeria.
Molecules. 2024 Dec 16;29(24):5935. doi: 10.3390/molecules29245935.
Twelve compounds (-), kaempferol (), luteolin (), luteolin 4'--xyloside (), luteolin 4'--β-glucoside (), quercetin 4'--β-xyloside (), kaempferol-3--[6″--(E)-p-coumaroyl]-β-D-glucoside (-tiliroside) (), protocatechuic acid (), gallic acid (), methyl gallate (), ethyl gallate (), shikimic acid-3--gallate (), and 3,3',4'-tri--methyl-ellagic acid 4-sulfate (), were isolated and identified from the aerial parts of (Cav.) Pers (synonym: C. Presl. All compounds were isolated by applying different chromatographic procedures, such as silica gel, RP-18 and Sephadex LH-20 columns. The structures were elucidated by extensive spectroscopic methods, mainly nuclear magnetic resonance NMR 1D and 2D, and mass spectrometry, as well as by comparison with the reported spectroscopic data. The two organic extracts, ethyl acetate (EtOAc) and butanol (BuOH), were evaluated for their potent phenolic and flavonoid contents using the Folin-Ciocalteu and aluminum chloride colorimetric methods. Furthermore, the antioxidant activity of the two extracts was determined using the DPPH, FRAP, and ABTS methods. Pure -tiliroside (), the main isolated compound, and luteolin 4'--β-xyloside () were evaluated for their antitumor activity against the lung cancer (A549), melanoma (A375) and pancreatic cancer (Mia PaCa-2 and Panc-1) cell lines by MTT assay.
从（Cav.）Pers（同义词：C. Presl）的地上部分分离并鉴定出12种化合物，分别是山柰酚（-）、木犀草素（）、木犀草素4'- -木糖苷（）、木犀草素4'- -β-葡萄糖苷（）、槲皮素4'- -β-木糖苷（）、山柰酚-3- -[6″- -（E）-对香豆酰基]-β-D-葡萄糖苷（-tiliroside）（）、原儿茶酸（）、没食子酸（）、没食子酸甲酯（）、没食子酸乙酯（）、莽草酸-3- -没食子酸酯（）和3,3',4'-三- -甲基-鞣花酸4-硫酸盐（）。所有化合物均通过硅胶、RP-18和Sephadex LH-20柱等不同色谱方法进行分离。通过广泛的光谱方法，主要是一维和二维核磁共振（NMR）以及质谱，并与报道的光谱数据进行比较来阐明结构。使用福林-酚试剂法和氯化铝比色法对乙酸乙酯（EtOAc）和丁醇（BuOH）这两种有机提取物的酚类和黄酮类含量进行了评估。此外，使用DPPH、FRAP和ABTS方法测定了这两种提取物的抗氧化活性。通过MTT法评估了主要分离化合物纯 -tiliroside（）以及木犀草素4'- -β-木糖苷（）对肺癌（A549）、黑色素瘤（A375）和胰腺癌（Mia PaCa-2和Panc-1）细胞系的抗肿瘤活性。
