Facultad de Ciencias Agrarias, Universidad Nacional Santiago Antúnez de Mayolo, Av. Centenario 200, 02002 Independencia, Ancash Huaraz, Peru.
Centro de Investigación de la Biodiversidad y Recursos Genéticos, Facultad de Ciencias, Universidad Nacional Santiago Antúnez de Mayolo, Av. Centenario 200, 02002 Independencia, Ancash, Huaraz, Peru.
Microbiol Res. 2020 Mar;233:126413. doi: 10.1016/j.micres.2020.126413. Epub 2020 Jan 15.
Microbial diversity in Peruvian mountain areas is poorly know, specially endophytic microorganisms of medicinal native plants from the Cordillera Blanca. So, nine bacterial and six fungal species were isolated from Gentianella weberbaueri and Valeriana pycnantha. According to 16S rDNA analysis, bacterial strains belong to genera Rahnella, Pseudomonas, Serratia, Rouxiella, and Bacillus; while ITS analysis showed that fungi belong to Pyrenochaeta, Scleroconidioma, Cryptococcus, and Plenodomus genera. Rahnella sp. GT24B and P. trivialis VT20B solubilized tricalcium phosphate and produced siderophores at 10 and 24 °C. Five bacteria strains produced indol-3-acetic acid (IAA) at 10 and 24 °C, where Rahnella sp. VT19B showed more production at 10 °C than 24 °C. Rahnella sp. GT24B, Serratia sp. VT28B, and Rahnella sp. GT25B inhibited Fusarium oxysporum growth up to 100, 78 and 74 %, respectively. R. inusitata VT25B and B. licheniformis GT10B showed high cellulolytic and proteolytic activities. On the other hand, only a few fungi moderately inhibited growth of F. oxysporum, and produced siderophores and cellulases. Most of bacteria inoculated on Medicago sativa "alfalfa" and Triticum aestivum "wheat" seeds got better root development, especially Rahnella sp. GT24B, Rouxiella sp.VT24B, Serratia sp. VT28B, and Rahnella sp. VT34B. Finally, this study is the first report of endophytic microorganisms associated to wild medicinal high-mountain Peruvian plants and it show a valuable microbial diversity and its possible role in promoting growth of crops and wild medicinal plants.
秘鲁山区的微生物多样性知之甚少，特别是来自白岭山脉的药用本土植物的内生微生物。因此，从 Gentianella weberbaueri 和 Valeriana pycnantha 中分离出了九种细菌和六种真菌。根据 16S rDNA 分析，细菌菌株属于 Rahnella、Pseudomonas、Serratia、Rouxiella 和 Bacillus 属；而 ITS 分析表明，真菌属于 Pyrenochaeta、Scleroconidioma、Cryptococcus 和 Plenodomus 属。Rahnella sp. GT24B 和 P. trivialis VT20B 在 10 和 24°C 下溶解磷酸三钙并产生铁载体。五种细菌菌株在 10 和 24°C 下产生吲哚-3-乙酸（IAA），其中 Rahnella sp. VT19B 在 10°C 下的产量高于 24°C。Rahnella sp. GT24B、Serratia sp. VT28B 和 Rahnella sp. GT25B 分别抑制 Fusarium oxysporum 的生长高达 100%、78%和 74%。R. inusitata VT25B 和 B. licheniformis GT10B 表现出较高的纤维素酶和蛋白酶活性。另一方面，只有少数真菌对 F. oxysporum 的生长有中度抑制作用，并产生铁载体和纤维素酶。接种在 Medicago sativa“紫花苜蓿”和 Triticum aestivum“小麦”种子上的大多数细菌都能更好地促进根的发育，特别是 Rahnella sp. GT24B、Rouxiella sp. VT24B、Serratia sp. VT28B 和 Rahnella sp. VT34B。最后，本研究首次报道了与秘鲁高山野生药用植物相关的内生微生物，展示了有价值的微生物多样性及其在促进作物和野生药用植物生长方面的可能作用。
