Negi Rajeshwari, Kaur Tanvir, Devi Rubee, Kour Divjot, Yadav Ajar Nath
Microbial Biotechnology Laboratory, Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, 173101, Himachal Pradesh, India.
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour, 173101, Himachal Pradesh, India.
Heliyon. 2022 Dec 25;8(12):e12579. doi: 10.1016/j.heliyon.2022.e12579. eCollection 2022 Dec.
Microbes play crucial functions in maintaining the health and growth of the plants directly or indirectly by supplying nutrients. These microbes could be used as biofertilizers for the enhancement of soil health and growth of crops. In preset investigation, potential microbes from endophytic and rhizospheric region of growing in green slopes of Shivaliks, Himachal Pradesh were sorted out and screened for plant growth promoting attributes including phosphorus and potassium solubilization. The potential bacterial strains were identified through 16S rRNA gene sequencing and developed as microbial consortium for the plant growth of wheat and wild wheat relative . A total 125 isolates of bacteria were sorted out and among them 36 were found as P-solubilizers and 19 showed K-solubilization attribute and two highly potential bacterial strain were identified as EU-ARP-44 (P-solubilizer; 270.5 ± 0.00 mg L) and EU-ARK-23 (K-Solubilizer; 51.3 ± 1.7 mg mL). The microbial consortium of sp. strain EU-A3SNfb (N-fixer; MN294545), EU-ARP-44 (P-solubilizer) and EU-ARK-23 (K-solubilizer) evaluation in and wheat crop resulted in the enhancement of growth as well as physiological parameter including shoot/root length, fresh/dry weight and chlorophyll, carotenoid, total soluble sugar content, phenolic and flavonoid content as compared to un-inoculated control. Microbial consortium consisting of potential plant growth promoting (PGP) bacterial strains could be used as biofertilizer and bioinoculants in cereals crop growing in hilly region.
微生物通过提供养分直接或间接地在维持植物的健康和生长方面发挥着关键作用。这些微生物可用作生物肥料，以增强土壤健康和作物生长。在本次调查中，从喜马偕尔邦西瓦利克山脉绿色山坡上生长的植物内生和根际区域筛选出潜在微生物，并对其促进植物生长的特性进行筛选，包括磷和钾的溶解。通过16S rRNA基因测序鉴定潜在细菌菌株，并将其开发为微生物联合体，用于小麦和野生小麦近缘种的植物生长。共筛选出125株细菌分离株，其中36株为磷溶解菌，19株表现出钾溶解特性，鉴定出两株高潜力细菌菌株，分别为EU-ARP-44（磷溶解菌；270.5±0.00毫克/升）和EU-ARK-23（钾溶解菌；51.3±1.7毫克/毫升）。对sp.菌株EU-A3SNfb（固氮菌；MN294545）、EU-ARP-44（磷溶解菌）和EU-ARK-23（钾溶解菌）的微生物联合体在小麦作物上进行评估，结果表明与未接种对照相比，其生长以及生理参数包括地上部/根长、鲜重/干重以及叶绿素、类胡萝卜素、总可溶性糖含量、酚类和黄酮类含量均有所提高。由潜在促进植物生长（PGP）细菌菌株组成的微生物联合体可作为生物肥料和生物接种剂用于丘陵地区种植的谷类作物。
