吸入氯乙烯后小鼠肝脏DNA中单链断裂的诱导

Induction of single-strand breaks in liver DNA of mice after inhalation of vinyl chloride.

作者信息

Walles S A, Holmberg B, Svensson K, Osterman-Golkar S, Sigvardsson K, Lindblom K

机构信息

National Institute of Occupational Health, Solna, Sweden.

出版信息

IARC Sci Publ. 1988(89):227-31.

PMID:3198205

Abstract

NMRI female mice were exposed to 100, 250 and 500 ppm vinyl chloride (VC). Cell nuclei were prepared from the liver, and single-strand breaks (SSB) were determined by the DNA unwinding technique. Haemoglobin (Hb) was isolated from the blood, and the degree of alkylation was determined as a measure of in-vivo dose by means of a gas chromatography-mass spectrometry (GC-MS) technique. A maximum level of SSB in liver DNA and of adduct levels of Hb was reached at 500 ppm, indicating that saturation of metabolic activation of VC had been achieved. The results demonstrate that VC induces SSB in liver DNA of mice in a dose-dependent manner and that about 80% of the damage is repaired within 20 h.

摘要

将NMRI雌性小鼠暴露于100、250和500 ppm的氯乙烯（VC）中。从肝脏中制备细胞核，并通过DNA解旋技术测定单链断裂（SSB）。从血液中分离出血红蛋白（Hb），并通过气相色谱 - 质谱（GC-MS）技术测定烷基化程度，以此作为体内剂量的衡量指标。在500 ppm时达到了肝脏DNA中SSB的最高水平以及Hb加合物水平，这表明已实现VC代谢活化的饱和。结果表明，VC以剂量依赖性方式诱导小鼠肝脏DNA中的SSB，并且约80%的损伤在20小时内得到修复。