Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, South Korea.
Department of Bioscience and Biotechnology, Microbial Carbohydrate Resource Bank (MCRB), Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, South Korea; Institute for Ubiquitous Information Technology and Applications (UBITA), Center for Biotechnology Research in UBITA (CBRU), Konkuk University, Seoul 05029, South Korea.
Int J Biol Macromol. 2020 Apr 15;149:281-289. doi: 10.1016/j.ijbiomac.2020.01.228. Epub 2020 Jan 23.
Pure gelatin hydrogel (PG) has limited practical applications due to their thermal instability and unfavorable mechanical properties. To overcome these limitations, dually crosslinked hydrogels were developed by imparting chemical crosslinking to existing physically crosslinked gelatin hydrogel networks using succinoglycan dialdehyde (SGDA) as a macromolecular crosslinker. SGDA-reinforced gelatin hydrogels (SGDA/Gels) displayed an 11 times higher compressive stress under identical deformation strain and a 1040% improvement in storage modulus (G') than PG. In addition, chemical crosslinking induced by SGDA increased the thermal stability of SGDA/Gels, such that they did not decompose at 60 °C, as confirmed by oscillatory temperature ramp experiments. The newly synthesized SGDA/Gels with reinforced networks and thermal stability exhibit potential for long-term use as controlled drug delivery carriers and 3D cell culture scaffolds for tissue engineering.
纯明胶水凝胶（PG）由于其热不稳定性和不利的机械性能，限制了其实际应用。为了克服这些限制，通过用琥珀酰基低聚糖醛（SGDA）作为大分子交联剂对现有物理交联的明胶水凝胶网络进行化学交联，开发了双重交联水凝胶。SGDA 增强的明胶水凝胶（SGDA/Gels）在相同的变形应变下表现出 11 倍更高的压缩应力，储能模量（G'）提高了 1040%，优于 PG。此外，SGDA 诱导的化学交联提高了 SGDA/Gels 的热稳定性，使其在 60°C 下不会分解，这一点通过振荡升温实验得到了证实。新合成的具有增强网络和热稳定性的 SGDA/Gels 有望作为长期使用的控制药物输送载体和用于组织工程的 3D 细胞培养支架。
