尼泊尔出现同时携带 bla 和 bla 的高度耐碳青霉烯类肺炎克雷伯菌临床分离株。
Emergence of clinical isolates of highly carbapenem-resistant Klebsiella pneumoniae co-harboring bla and bla in Nepal.
机构信息
Department of Medical Microbiology, Kathmandu University School of Medical Sciences, Dhulikhel, Nepal.
Department of Microbiology, Juntendo University Graduate School of Medicine, Tokyo, Japan.
出版信息
Int J Infect Dis. 2020 Mar;92:247-252. doi: 10.1016/j.ijid.2020.01.040. Epub 2020 Jan 23.
OBJECTIVES
The aim of this study was to describe the emergence in Nepal of clinical isolates of Klebsiella pneumoniae harboring both bla and bla.
METHODS
Six clinical isolates of K. pneumoniae highly resistant to carbapenems and aminoglycosides were obtained from inpatients in Nepal. Their whole genomes were sequenced by a next generation sequencer.
RESULTS
The minimum inhibitory concentrations of meropenem, amikacin and ciprofloxacin were ≥128 μg/ml, >1024 μg/ml and ≥256 μg/ml, respectively. All six isolates co-harbored bla, bla and rmtB. Of them, 1 also harbored rmtF. The bla, bla and rmtB in all six isolates were located on plasmids. Of the six isolates tested, one isolate harbored two copies of bla and rmtF on the chromosome.
CONCLUSIONS
This is the first report of clinical isolates of K. pneumoniae co-harboring bla, bla and rmtB in Nepal. These strains were highly carbapenem- and aminoglycoside-resistant, and belonged to ST147 or ST395. Of them, ST147 isolate harbored two copies of bla on the chromosome.
目的
本研究旨在描述在尼泊尔出现同时携带 bla 和 bla 的肺炎克雷伯菌临床分离株。
方法
从尼泊尔住院患者中获得了 6 株对碳青霉烯类和氨基糖苷类高度耐药的肺炎克雷伯菌临床分离株。使用下一代测序仪对它们的全基因组进行测序。
结果
美罗培南、阿米卡星和环丙沙星的最低抑菌浓度分别≥128μg/ml、>1024μg/ml 和≥256μg/ml。所有 6 株分离株均同时携带 bla、bla 和 rmtB。其中 1 株还携带 rmtF。bla、bla 和 rmtB 均位于质粒上。在检测的 6 株分离株中，有 1 株携带 bla 和 rmtF 的两个拷贝位于染色体上。
结论
这是尼泊尔首次报告同时携带 bla、bla 和 rmtB 的肺炎克雷伯菌临床分离株。这些菌株对碳青霉烯类和氨基糖苷类高度耐药，属于 ST147 或 ST395。其中，ST147 分离株携带 bla 的两个拷贝位于染色体上。
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