台湾南部 Zika 与登革热感染之免疫交互作用及其流行现况之流行病学调查。

An epidemiological survey of the current status of Zika and the immune interaction between dengue and Zika infection in Southern Taiwan.

机构信息

Center for Tropical Medicine and Infectious Disease, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan; Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.

Center for Tropical Medicine and Infectious Disease, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan; Program in Tropical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 80708, Taiwan.

出版信息

Int J Infect Dis. 2020 Apr;93:151-159. doi: 10.1016/j.ijid.2020.01.031. Epub 2020 Jan 23.

DOI:10.1016/j.ijid.2020.01.031

PMID:31982624

Abstract

OBJECTIVES

This study was performed to examine the current status of Zika and the effects of pre-existing dengue immunity on Zika virus (ZIKV) infection in Southern Taiwan.

METHODS

A phylogenetic tree was used to analyze the phylogeny of detected ZIKVs. Paired sera from dengue patients were collected for the determination of dengue and Zika infection. Plaque reduction neutralization tests (PRNT) and quantitative reverse transcription PCR (qRT-PCR) were used to determine the titers of neutralizing antibodies and viruses, respectively. An antibody-dependent enhancement (ADE) assay was used to evaluate the effect of anti-dengue antibodies on ZIKV infection.

RESULTS

Epidemiological data indicated the continuous importation of ZIKV infection from neighboring Zika epidemic countries into Taiwan. A total of 78 dengue patients were enrolled and 21 paired serum samples were obtained. PRNT results for the 21 samples identified eight cases of primary dengue infection and 13 cases of secondary dengue infection; two samples were positive for ZIKV (MR766). Results from the ADE assay indicated that convalescent sera from primary and secondary dengue infection patients displayed significant ADE of the ZIKV infection when compared to healthy controls (p < 0.05).

CONCLUSIONS

This study suggests that pre-existing dengue immunity facilitates ZIKV infection and that the continuous importation of ZIKV infection may pose a threat to indigenous Zika emergence in Southern Taiwan.

摘要

目的

本研究旨在调查寨卡病毒在台湾南部的流行现状，以及既往登革热感染对寨卡病毒（ZIKV）感染的影响。

方法

通过构建进化树分析检测到的寨卡病毒的进化关系。收集登革热患者的配对血清，以确定登革热和寨卡病毒感染情况。采用空斑减少中和试验（PRNT）和实时定量逆转录 PCR（qRT-PCR）分别检测中和抗体滴度和病毒载量。采用抗体依赖性增强（ADE）试验评估抗登革热抗体对寨卡病毒感染的影响。

结果

流行病学数据表明，寨卡病毒持续从邻近的寨卡流行国家输入台湾。共纳入 78 例登革热患者，获得 21 对配对血清样本。21 份样本的 PRNT 结果鉴定出 8 例原发性登革热和 13 例继发性登革热；有 2 份样本对 ZIKV（MR766）呈阳性。ADE 试验结果表明，与健康对照组相比，原发性和继发性登革热感染患者的恢复期血清对 ZIKV 感染具有显著的 ADE（p＜0.05）。