Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia.
Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; Botany and Microbiology Department, Faculty of Science, University of Beni-Suef, Beni-Suef, Egypt.
J Photochem Photobiol B. 2020 Mar;204:111786. doi: 10.1016/j.jphotobiol.2020.111786. Epub 2020 Jan 10.
Biological synthesis of nanoparticles is a growing research trend because it has numerous applications in pharmaceutics and biomedicine. The aim of this study was to obtain silver nanoparticles (AgNPs) from Bacillus sp. KFU36, a marine strain, and to assess its anticancer activity. The supernatant of Bacillus sp. KFU36 was supplemented with silver nitrate and the nanoparticles obtained were characterized spectrophotometrically and microscopically. A band of surface plasmon resonance was appeared at 430 nm, as revealed by UV-vis spectrophotometry. X-ray diffraction spectrum and Energy Dispersive Spectroscopy confirmed the crystalline and metallic structure of the AgNPs, respectively. Scanning electron microscopy revealed that the shape of the synthesized AgNPs were spherical and the size extended between 5 and 15 nm. The AgNPs were investigated for their potential anticancer effects on the cell viability, migration and apoptosis using MTT and wound-healing assays, and flow cytometry, respectively. The cytotoxic effects of these nanoparticles were evidenced by the decreasing the cell viability (as 15% at 50 μg/ml), cell density, adhesion capacity and losing the normal shape and size, and inducing the apoptosis on MCF-7 by 61% at 50 μg/ml. These findings confirm that the synthesized AgNPs exhibited superior anticancer activities and therefore could be exploited as a promising, cost-effective, and environmentally benign strategy in treating this disease in future.
纳米粒子的生物合成是一个不断发展的研究趋势，因为它在药剂学和生物医学中有许多应用。本研究的目的是从海洋菌株 Bacillus sp. KFU36 中获得银纳米粒子（AgNPs），并评估其抗癌活性。在 Bacillus sp. KFU36 的上清液中补充硝酸银，并用分光光度法和显微镜对获得的纳米粒子进行特征描述。紫外可见分光光度法显示，表面等离子体共振带出现在 430nm 处。X 射线衍射光谱和能谱分别证实了 AgNPs 的结晶和金属结构。扫描电子显微镜显示，合成的 AgNPs 的形状为球形，尺寸在 5 到 15nm 之间。通过 MTT 和划痕实验分别研究了 AgNPs 对细胞活力、迁移和凋亡的潜在抗癌作用，以及流式细胞术。这些纳米粒子的细胞毒性作用通过降低细胞活力（在 50μg/ml 时为 15%）、细胞密度、粘附能力以及失去正常形状和大小，并在 50μg/ml 时诱导 MCF-7 细胞凋亡 61%得到证实。这些发现证实，合成的 AgNPs 表现出优异的抗癌活性，因此可以在未来作为一种有前途、具有成本效益和环境友好的治疗这种疾病的策略加以利用。
