Briquet M
Biochim Biophys Acta. 1977 Feb 7;459(2):290-9. doi: 10.1016/0005-2728(77)90029-9.
Evidence for the existence of mediated transport of pyruvate and lactate in isolated mitochondria of Saccharomyces cerevisiae is presented. 1. The mitochondrial oxidation of pyruvate is specifically inhibited by the monocarboxylic oxoacids alpha-ketoisocaproate and by alpha-cyano-3-hydroxycinnamate, while pyruvate and malate dehydrogenases activities are not inhibited. 2. The stimulation of the mitochondrial oxidations of succinate, alpha-ketoglutarate and citrate by pyruvate are also inhibited by alpha-cyano-3-hydroxycinnamate. 3. The [14C]pyruvate uptake by yeast mitochondria follows saturation kinetics and is completely inhibited by alpha-cyano-3-hydroxycinnamate. 4. Large amplitude passive swellings of mitochondria of the wild type and of cytoplasmic rho- and rho-n mutants are induced by isoosmotic ammonium pyruvate and lactate. These pH-dependent swellings are inhibited by alpha-cyano-3-hydroxycinnamate suggesting that the carrier system is not coded by mitochondrial DNA.
本文提供了酿酒酵母分离线粒体中丙酮酸和乳酸介导转运存在的证据。1. 丙酮酸的线粒体氧化被一元羧酸α-酮异己酸和α-氰基-3-羟基肉桂酸特异性抑制，而丙酮酸和苹果酸脱氢酶活性未受抑制。2. α-氰基-3-羟基肉桂酸也抑制丙酮酸对琥珀酸、α-酮戊二酸和柠檬酸线粒体氧化的刺激作用。3. 酵母线粒体对[14C]丙酮酸的摄取遵循饱和动力学，且被α-氰基-3-羟基肉桂酸完全抑制。4. 等渗丙酮酸铵和乳酸可诱导野生型以及细胞质rho-和rho-n突变体线粒体的大幅度被动肿胀。这些pH依赖性肿胀被α-氰基-3-羟基肉桂酸抑制，表明载体系统不是由线粒体DNA编码的。
